US 12,258,547 B2
Cell culture plate, method for preparing thereof and method for detecting a substance
Markus Nuopponen, Helsinki (FI); Lauri Paasonen, Järvenpää (FI); Marika Mannerström, Turku (FI); Tuula Heinonen, Espoo (FI); and Tarja Toimela, Pirkkala (FI)
Assigned to UPM-KYMMENE CORPORATION, Helsinki (FI)
Filed by UPM-Kymmene Corporation, Helsinki (FI)
Filed on May 19, 2020, as Appl. No. 16/878,044.
Claims priority of application No. 19397521 (EP), filed on Jun. 10, 2019.
Prior Publication US 2020/0385660 A1, Dec. 10, 2020
Int. Cl. C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/00 (2006.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01)
CPC C12M 23/12 (2013.01) [C12M 23/34 (2013.01); C12M 25/06 (2013.01); C12N 5/0018 (2013.01); C12Q 1/025 (2013.01); G01N 33/502 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/78 (2013.01); G01N 2500/10 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A cell culture plate comprising one or more cell culture well(s) comprising at least one barrier for dividing the well into at least two compartments, wherein the barrier comprises nanofibrillar cellulose hydrogel, wherein the nanofibrillar cellulose has an average fibril diameter of less than 200 nanometers, wherein the nanofibrillar cellulose hydrogel comprises 0.8-2.5% (w/w) of nanofibrillar cellulose, wherein the wall thickness of the barrier is in the range of 0.5-2 mm, wherein cells cannot penetrate the barrier, and wherein the barrier has a height of 1 to 6 mm.