US 12,258,477 B2
Silicon-substituted rhodamine dyes and dye conjugates
Jongtae Yang, Eugene, OR (US); Xin Wang, Eugene, OR (US); Yi-Zhen Hu, Eugene, OR (US); Kyle Gee, Springfield, OR (US); Aimei Chen, Eugene, OR (US); and Aleksey Rukavishnikov, Eugene, OR (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Appl. No. 17/150,247
Filed by Life Technologies Corporation, Carlsbad, CA (US)
PCT Filed Aug. 8, 2019, PCT No. PCT/US2019/045697
§ 371(c)(1), (2) Date Jan. 15, 2021,
PCT Pub. No. WO2020/033681, PCT Pub. Date Feb. 13, 2020.
Claims priority of provisional application 62/717,398, filed on Aug. 10, 2018.
Prior Publication US 2021/0324198 A1, Oct. 21, 2021
Int. Cl. C09B 11/28 (2006.01); C07F 7/08 (2006.01); C07F 9/6561 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6869 (2018.01); G01N 33/58 (2006.01)
CPC C09B 11/28 (2013.01) [C07F 7/0816 (2013.01); C07F 9/6561 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); G01N 33/582 (2013.01)] 7 Claims
 
1. A compound of formula (I), or a spirolactone form and/or salt thereof:

OG Complex Work Unit Chemistry
wherein:
R1 is methyl or C2-6 alkyl or -L1-RB1;
R2 is methyl, C2-6 alkyl, or -L1-RB1;
L1 is a linker comprising a combination of two or more groups selected from alkyl, —C(O)NH—, —S—, —O—, and/or —S(O)—;
R3 is —COOH, CO2−, or SO3−;
each of E, R4, R5, R6, R7, R8, and R9 is H;
each RN1 is independently H, methyl, or C2-4 alkyl;
each RN2 is independently H, methyl, or C2-4 alkyl;
provided that
(i) at least one of R1 or R2 is -L1-RB1, and the RB1 group comprises a nucleic acid-binding moiety or a cytoskeleton-binding moiety,
wherein the nucleic acid-binding moiety or the cytoskeleton-binding moiety is selected from

OG Complex Work Unit Chemistry
where the wavy line indicates a connection to L1.