US 12,258,430 B2
Compositions and methods for labeling and modulation of cells in vitro and in vivo
Hua Wang, Cambridge, MA (US); and David J. Mooney, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Mar. 18, 2021, as Appl. No. 17/206,050.
Application 17/206,050 is a continuation of application No. PCT/US2019/051621, filed on Sep. 18, 2019.
Claims priority of provisional application 62/733,378, filed on Sep. 19, 2018.
Prior Publication US 2021/0284776 A1, Sep. 16, 2021
Int. Cl. C08F 251/00 (2006.01); A61K 39/00 (2006.01); A61K 49/22 (2006.01); A61N 7/00 (2006.01); A61P 35/00 (2006.01); C08B 37/00 (2006.01); G01N 33/543 (2006.01); G01N 33/548 (2006.01)
CPC C08F 251/00 (2013.01) [A61K 39/0011 (2013.01); A61K 49/221 (2013.01); A61K 49/222 (2013.01); A61N 7/00 (2013.01); A61P 35/00 (2018.01); C08B 37/0006 (2013.01); G01N 33/54346 (2013.01); G01N 33/548 (2013.01); A61N 2007/0004 (2013.01); C08F 2438/03 (2013.01)] 18 Claims
 
1. A click functionalized polysaccharide polymer which is a product of radical-catalyzed polymerization;
wherein said radical-catalyzed polymerization involves a reaction between one or more saccharide monomers;
wherein each saccharide monomer comprises a saccharide molecule; a click reagent attached to said saccharide molecule; and a moiety comprising a functional group amenable to radical polymerization attached to said saccharide molecule; and
wherein:
(i) said saccharide molecule further comprises one or more hydrolysable substituents at the C1 position, the C3 position, the C4 position or C5 position, and wherein at least one of said hydrolysable substituent is represented by formula (1):

OG Complex Work Unit Chemistry
wherein R is alkyl;
(ii) said moiety comprising a functional group amenable to radical polymerization is attached to said saccharide molecule at the C1 position;
(iii) said moiety comprising a functional group amenable to radical polymerization comprises an acrylate or methacrylate; and/or
(iv) said radical-catalyzed polymerization is reversible addition-fragmentation chain transfer (RAFT) polymerization involving the use of a RAFT agent; and wherein said RAFT agent comprises a thiocarbonate moiety, a dithiocarbamate moiety or a dithiobenzoate moiety.