	US 12,258,385 B2
	CD137 binding fibronectin type III domains
Michael Diem, Havertown, PA (US); Rebecca Hawkins, Harleysville, PA (US); Steven Jacobs, North Wales, PA (US); and Manuel Sepulveda, Princeton Junction, NJ (US)
Assigned to Janssen Biotech, Inc., Horsham, PA (US)
Filed by JANSSEN BIOTECH, INC, Horsham, PA (US)
Filed on Apr. 28, 2022, as Appl. No. 17/732,420.
Application 17/732,420 is a continuation of application No. 16/820,844, filed on Mar. 17, 2020, granted, now 11,345,739.
Application 16/820,844 is a continuation of application No. 15/840,303, filed on Dec. 13, 2017, granted, now 10,611,823, issued on Apr. 7, 2020.
Claims priority of provisional application 62/434,064, filed on Dec. 14, 2016.
Prior Publication US 2022/0259289 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/78 (2006.01); A61P 35/00 (2006.01); C07K 14/705 (2006.01); C12N 15/85 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/574 (2006.01); G01N 33/68 (2006.01); A61K 38/39 (2006.01); C07K 14/71 (2006.01)

CPC C07K 14/78 (2013.01) [A61P 35/00 (2018.01); C07K 14/705 (2013.01); C07K 14/70596 (2013.01); C12N 15/85 (2013.01); G01N 33/53 (2013.01); G01N 33/574 (2013.01); G01N 33/57492 (2013.01); G01N 33/6893 (2013.01); A61K 38/39 (2013.01); C07K 2319/31 (2013.01); C07K 2319/40 (2013.01)]

9 Claims

1. A method of detecting CD137-expressing cancer cells in a tumor tissue, comprising:

conjugating an isolated protein comprising an amino acid sequence that is at least 90% identical to a sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 48, 49, 50, 51, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, and 224 to a detectable label of to form a conjugate;

administering the conjugate to a subject; and

visualizing the CD137-expressing cancer cells to which the conjugate is bound.