US 12,258,375 B2
Mutant lysenin pores
Mark John Bruce, Oxford (GB); James Anthony Clarke, Oxford (GB); Andrew John Heron, Oxford (GB); Lakmal Nishantha Jayasinghe, Oxford (GB); and Elizabeth Jayne Wallace, Oxford (GB)
Assigned to Oxford Nanopore Technologies PLC, Oxford (GB)
Filed by Oxford Nanopore Technologies PLC, Oxford (GB)
Filed on Nov. 8, 2023, as Appl. No. 18/504,590.
Application 18/504,590 is a continuation of application No. 17/108,536, filed on Dec. 1, 2020, granted, now 11,845,780.
Application 17/108,536 is a continuation of application No. 15/692,498, filed on Aug. 31, 2017, granted, now 10,882,889.
Application 15/692,498 is a continuation of application No. 14/391,660, granted, now 9,777,049, previously published as PCT/GB2013/050667, filed on Mar. 15, 2013.
Claims priority of provisional application 61/622,174, filed on Apr. 10, 2012.
Prior Publication US 2024/0199711 A1, Jun. 20, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/435 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); G01N 27/447 (2006.01)
CPC C07K 14/43536 (2013.01) [G01N 27/447 (2013.01); C07K 2319/55 (2013.01); C12Q 1/6869 (2013.01)] 20 Claims
 
1. A method of characterising a target analyte, comprising:
(a) contacting the target analyte with a protein pore such that the target analyte moves through the protein pore, wherein the protein pore comprises at least one lysenin monomer that comprises a barrel-forming region comprising the amino acid sequence as set forth in positions 44 to 126 of SEQ ID NO: 2 with one or more amino acid substitutions, wherein at least one of the amino acid substitutions introduces a cysteine, lysine, or non-natural amino acid; and
(b) taking one or more measurements as the target analyte moves through the protein pore, wherein the measurements are indicative of one or more characteristics of the target analyte, thereby characterising the target analyte.