US 12,258,369 B2
Compositions and methods for synthesizing 5′-capped RNAs
Richard I. Hogrefe, San Diego, CA (US); Alexandre Lebedev, San Diego, CA (US); Anton P. McCaffrey, San Diego, CA (US); and Dongwon Shin, San Diego, CA (US)
Assigned to TriLink BioTechnologies, LLC, San Diego, CA (US)
Filed by TriLink BioTechnologies, LLC, San Diego, CA (US)
Filed on Sep. 14, 2023, as Appl. No. 18/467,224.
Application 18/467,224 is a continuation of application No. 17/091,422, filed on Nov. 6, 2020, granted, now 11,878,991.
Application 17/091,422 is a continuation of application No. 15/761,957, granted, now 10,913,768, issued on Feb. 9, 2021, previously published as PCT/US2016/052670, filed on Sep. 20, 2016.
Claims priority of provisional application 62/221,248, filed on Sep. 21, 2015.
Prior Publication US 2024/0140982 A1, May 2, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/02 (2006.01); A23L 33/13 (2016.01); A61K 31/711 (2006.01); A61K 31/7115 (2006.01); A61K 31/712 (2006.01); C12N 15/11 (2006.01); C12P 19/34 (2006.01)
CPC C07H 21/02 (2013.01) [A23L 33/13 (2016.08); A61K 31/711 (2013.01); A61K 31/7115 (2013.01); A61K 31/712 (2013.01); C12N 15/11 (2013.01); C12P 19/34 (2013.01); C12Y 207/07 (2013.01)] 29 Claims
 
1. An initiating capped oligonucleotide primer comprising the following structure:

OG Complex Work Unit Chemistry
wherein:
each of B1 through Bio is independently a natural, modified or unnatural nucleoside base;
M is 0 or 1;
L is 0 or 1;
q1 is 1 and each of q2 through q9 is independently 0 or 1;
each R1 is independently H or methyl;
R2 and R3 are independently H, OH, alkyl, O-alkyl, halogen, a linker or a detectable marker;
each of X1 through X13 is independently O or S;
each of Y1 through Y13 is independently OH, SH, BH3, aryl, alkyl, O-alkyl or O-aryl;
each of Z0 through Z22 is independently O, S, NH, CH2, C(halogen)2 or CH (halogen); and
each of R4 through R12 is independently H, OH, O-methyl, or a detectable marker, wherein:
B1 is complementary to a nucleoside base on a nucleic acid template at transcription template position +1 from the transcription start site,
B2 through B9, if present, are complementary to the respective nucleoside bases on the nucleic acid template at transcription template position +2 and so on from the transcription start site, and
B10, the last nucleoside base of initiating capped oligonucleotide primer, is complementary to the last hybridized transcription template nucleotide.