US 12,258,368 B2
Disulfide-linked reversible terminators
Moti Jain, Palo Alto, CA (US); and Wei Zhou, Palo Alto, CA (US)
Assigned to CENTRILLION TECHNOLOGY HOLDINGS CORPORATION, Grand Cayman (KY)
Appl. No. 17/278,958
Filed by CENTRILLION TECHNOLOGY HOLDINGS CORPORATION, Grand Cayman (KY)
PCT Filed Sep. 27, 2019, PCT No. PCT/US2019/053629
§ 371(c)(1), (2) Date Mar. 23, 2021,
PCT Pub. No. WO2020/069424, PCT Pub. Date Apr. 2, 2020.
Claims priority of provisional application 62/847,458, filed on May 14, 2019.
Claims priority of provisional application 62/835,007, filed on Apr. 17, 2019.
Claims priority of provisional application 62/808,413, filed on Feb. 21, 2019.
Claims priority of provisional application 62/738,627, filed on Sep. 28, 2018.
Prior Publication US 2022/0048940 A1, Feb. 17, 2022
Int. Cl. C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/04 (2006.01); C12Q 1/6869 (2018.01); C07H 19/14 (2006.01); C07H 19/173 (2006.01)
CPC C07H 19/10 (2013.01) [C07H 21/04 (2013.01); C12Q 1/6869 (2013.01); C07H 19/14 (2013.01); C07H 19/173 (2013.01); C07H 19/20 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A nucleoside 5′-triphosphate analog according to formula (II):

OG Complex Work Unit Chemistry
or a salt or protonated form thereof, wherein:
X is O;
X′ is O or S;
w is 1;
base B is a nucleotide base or an analog thereof;
Rx is H or C1-6 alkyl, wherein the C1-6 alkyl is unsubstituted or substituted by 1-3groups
selected from the group consisting of F and Cl;
L1 is a first linker group and L1 is 3-25 atoms in length;
L2 is a second linker group and L2 is

OG Complex Work Unit Chemistry
and m is 2 or 3;
L3 is a third linker group and L3 is 4-47 atoms in length;
D1 is a fluorophore.