US 12,257,582 B2
Sample preliminary screening chip, specimen detecting method, and screening device
Hui Liao, Beijing (CN); Yingying Zhao, Beijing (CN); Wenliang Yao, Beijing (CN); Bolin Fan, Beijing (CN); Nan Zhao, Beijing (CN); Le Gu, Beijing (CN); and Yongjia Gao, Beijing (CN)
Assigned to Beijing BOE Sensor Technology Co., Ltd., Beijing (CN); and BOE Technology Group Co., Ltd., Beijing (CN)
Filed by Beijing BOE Sensor Technology Co., Ltd., Beijing (CN); and BOE Technology Group Co., Ltd., Beijing (CN)
Filed on Jun. 11, 2020, as Appl. No. 16/898,516.
Claims priority of application No. 201911307664.1 (CN), filed on Dec. 18, 2019.
Prior Publication US 2021/0187507 A1, Jun. 24, 2021
Int. Cl. B01L 3/00 (2006.01); G01N 21/65 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502792 (2013.01); G01N 21/65 (2013.01); B01L 2200/143 (2013.01); B01L 2400/086 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A specimen detecting method, comprising:
controlling, by a data processor, a sample solution containing a specimen to be added into a preliminary screening inlet of a sample preliminary screening chip; wherein the sample preliminary screening chip comprises:
a first preliminary screening substrate and a second preliminary screening substrate fitted to each other;
a groove structure of the first preliminary screening substrate facing the second preliminary screening substrate;
wherein the groove structure forms a channel encapsulated between the first preliminary screening substrate and the second preliminary screening substrate, and the channel is provided with two ends; and
the preliminary screening inlet in one of the two ends of the channel, and a preliminary screening outlet in the other one of the two ends of the channel; wherein
the preliminary screening inlet and the preliminary screening outlet run through the second preliminary screening substrate; the groove structure comprises a first groove structure and a second groove structure, the first groove structure is provided with a first depth in a direction perpendicular to a plane on which the first preliminary screening substrate is located, and the second groove structure is provided with a second depth in the direction perpendicular to the plane on which the first preliminary screening substrate is located; the channel is a preliminary screening area; in a direction from the preliminary screening inlet to the preliminary screening outlet, the preliminary screening area is divided into a first preliminary screening sub-area and a second preliminary screening sub-area; the first groove structure forms the first preliminary screening sub-area and the second groove structure forms the second preliminary screening sub-area; and
the first depth is greater than a maximum particle diameter of the specimen contained in the sample solution, and the second depth is smaller than a minimum particle diameter of the specimen;
controlling, by the data processor, the sample solution in the preliminary screening inlet to enter the channel, and controlling, by the data processor, a part of the sample solution to flow successively through the first preliminary screening sub-area and the second preliminary screening sub-area, and to flow out from the preliminary screening outlet so as to store a liquid with the specimen in the first preliminary screening sub-area;
controlling, by the data processor, the liquid in the first preliminary screening sub-area to flow into a liquid storage tank of a microfluidic chip;
controlling, by the data processor, the microfluidic chip to separate micro-droplets from a liquid in the liquid storage tank, wherein the micro-droplets are independent and micro-sized, a size of each of the micro-droplets is the sum of a maximum particle diameter of the specimen and a preset value;
acquiring, by the data processor, Raman spectra corresponding to each of the micro-droplets; and
controlling, by the data processor, the microfluidic chip to move at least one micro-droplet, among the micro-droplets, with the specimen into a specimen storage tank according to the Raman spectra corresponding to each of the micro-droplets.