US 12,257,581 B2
Sample preparation cartridges and apparatuses
Manojkumar M. Nair, Burlington, MA (US); and John Frederick Regan, Boxford, MA (US)
Assigned to Meridian LGH Holdings 2, LLC, Dallas, TX (US)
Filed by Meridian LGH Holdings 2, LLC, Dallas, TX (US)
Filed on Mar. 19, 2024, as Appl. No. 18/609,031.
Application 18/609,031 is a continuation of application No. 17/626,885, granted, now 11,964,279, previously published as PCT/US2020/041875, filed on Jul. 14, 2020.
Claims priority of provisional application 62/874,212, filed on Jul. 15, 2019.
Prior Publication US 2025/0041858 A1, Feb. 6, 2025
Int. Cl. B01L 3/00 (2006.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01); G01N 33/543 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC B01L 3/502753 (2013.01) [C12N 1/06 (2013.01); C12N 15/1017 (2013.01); G01N 33/54326 (2013.01); B01L 2200/0605 (2013.01); B01L 2300/0681 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); G01N 2035/00564 (2013.01); G01N 2035/1032 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of determining organism viability in a sample, comprising:
receiving a first volume of the sample in a first lysis chamber, the first volume of the sample containing a first plurality of cells;
lysing the first plurality of cells in the first lysis chamber to release a first plurality of nucleic acids;
moving the first plurality of nucleic acids into a first nucleic acid capture chamber;
capturing the first plurality of nucleic acids on a first nucleic acid binding unit in the first nucleic acid capture chamber;
releasing the first plurality of nucleic acids from the first nucleic acid binding unit;
processing the first plurality of nucleic acids released from the first nucleic acid binding unit with a sample processing instrument at a first time point to determine a first target signal;
receiving a second volume of the sample in a second lysis chamber, the second volume of the sample containing a second plurality of cells;
lysing the second plurality of cells in the second lysis chamber to release a second plurality of nucleic acids;
moving the second plurality of nucleic acids into a second nucleic acid capture chamber;
capturing the second plurality of nucleic acids on a second nucleic acid binding unit in the second nucleic acid capture chamber;
releasing the second plurality of nucleic acids from the second nucleic acid binding unit;
processing the second plurality of nucleic acids released from the second nucleic acid binding unit with the sample processing instrument at a second time point to determine a second target signal, the second time point being after the first time point; and
determining whether the second target signal is larger than the first target signal,
wherein determining that the second target signal is larger than the first target signal indicates presence of live organisms in the sample.