US 12,257,580 B2
Microfluidic chip and manufacture method thereof, and cell separation and single-cell western blotting method
Xianting Ding, Shanghai (CN); Ainur Abula, Shanghai (CN); Ting Zhang, Shanghai (CN); and Haiyang Xie, Shanghai (CN)
Assigned to Water Bear Health Technology (Nantong) Co., LTD., Nantong (CN)
Filed by Water Bear Health Technology (Nantong) Co., LTD., Nantong (CN)
Filed on Sep. 30, 2021, as Appl. No. 17/490,716.
Prior Publication US 2023/0102204 A1, Mar. 30, 2023
Int. Cl. B01L 3/00 (2006.01); G01N 33/53 (2006.01); G01N 33/68 (2006.01)
CPC B01L 3/502753 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); G01N 33/5304 (2013.01); G01N 33/68 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/0887 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A microfluidic chip, comprising:
a first unit which comprises a channel for a cell sample to pass through and is configured to separate circulating tumor cells in the cell sample;
a first interlayer, the first unit is provided on the first interlayer;
a second interlayer, the first interlayer is provided on the second interlayer;
a gel layer which is provided in the second interlayer; and
a second unit, an inlet of which communicates with an outlet of the first unit, and the second unit is configured to capture single cells from the separated circulating tumor cells and subject the captured single cells to closed lysis; and the second unit is provided in the gel layer;
wherein the microfluidic chip is configured to implement the binding of a protein molecule of a single cell with an antibody in the gel layer after the single cell is lysed.