US 12,257,530 B2
Fc binding proteins with cysteine in the c-terminal helical region
Erik Fiedler, Halle/Saale (DE); and Ulrich Haupts, Halle/Salle (DE)
Assigned to Repligen Corporation, Waltham, MA (US)
Filed by Repligen Corporation, Waltham, MA (US)
Filed on Sep. 5, 2023, as Appl. No. 18/460,843.
Application 18/460,843 is a continuation of application No. 16/637,323, granted, now 11,779,860, previously published as PCT/EP2018/071232, filed on Aug. 6, 2018.
Claims priority of application No. PCT/EP2017/069976 (WO), filed on Aug. 7, 2017; application No. 18154731 (EP), filed on Feb. 1, 2018; and application No. 18163964 (EP), filed on Mar. 26, 2018.
Prior Publication US 2024/0091671 A1, Mar. 21, 2024
Int. Cl. B01D 15/38 (2006.01); B01J 20/32 (2006.01); C07K 1/22 (2006.01); C07K 14/31 (2006.01)
CPC B01D 15/3809 (2013.01) [B01J 20/3274 (2013.01); C07K 1/22 (2013.01); C07K 14/31 (2013.01)] 22 Claims
 
1. An affinity separation matrix comprising an Fc binding protein comprising one or more domains, wherein at least one domain comprises an amino acid sequence of SEQ ID NO: 3, or an amino acid sequence with at least 91% identity thereto, and wherein at least one amino acid a position corresponding to position 40, 42, 43, 46, 47, 49, 50, 51, 53, or 54 of SEQ ID NO: 3 is cysteine.