US 12,257,367 B2
Biomaterials for biological tissue repair
Koji Nagahama, Kobe (JP)
Assigned to KONAN GAKUEN, Kobe (JP)
Filed by KONAN GAKUEN, Kobe (JP)
Filed on Jul. 27, 2020, as Appl. No. 16/939,204.
Claims priority of application No. 2019-098693 (JP), filed on May 27, 2019.
Prior Publication US 2020/0405914 A1, Dec. 31, 2020
Int. Cl. A61L 27/52 (2006.01); A61L 27/20 (2006.01); A61L 27/38 (2006.01)
CPC A61L 27/52 (2013.01) [A61L 27/20 (2013.01); A61L 27/3804 (2013.01); A61L 27/3826 (2013.01); A61L 27/3873 (2013.01)] 16 Claims
 
1. A method for repairing a biological tissue, comprising:
applying to an affected site a biomaterial comprising
a water-soluble polymer having a reactive functional groups A; and
mesenchymal stem cells having tissue-regenerating capacity and having, on the surface of the cells, reactive functional groups B that can covalently bind to the reactive functional groups A,
wherein the biomaterial presents a hydrogel state when the reactive functional groups A covalently bind to the reactive functional groups B,
wherein the water-soluble polymer is a polysaccharide thickener, and
wherein the mesenchymal cells having tissue-regenerating capacity serve as cross-linking points of the hydrogel for chemically binding with the water-soluble polymer, wherein the affected site to which the biomaterial is applied is selected from the group consisting of (i) epithelial tissue, (ii) connective tissue adjacent to epithelial tissue, and (iii) skeletal muscle tissue.