	US 12,257,339 B2
	Phytocomplex and extract of a meristematic cell line selected from a plant belonging to the genus Rosa
Elena Sgaravatti, Padua (IT); Giovanna Pressi, Rubano (IT); and Flavia Guzzo, Verona (IT)
Assigned to AETHERA BIOTECH S.R.L., Camisano Vicentino (IT)
Appl. No. 17/441,224
Filed by AETHERA BIOTECH S.R.L., Camisano Vicentino (IT)
PCT Filed Mar. 20, 2020, PCT No. PCT/IB2020/052584 § 371(c)(1), (2) Date Sep. 20, 2021, PCT Pub. No. WO2020/188531, PCT Pub. Date Sep. 24, 2020.
Claims priority of application No. 102019000004105 (IT), filed on Mar. 21, 2019.
Prior Publication US 2022/0175654 A1, Jun. 9, 2022
Int. Cl. A61K 36/738 (2006.01); A61K 8/9783 (2017.01); A61K 9/00 (2006.01); A61Q 19/08 (2006.01)

CPC A61K 8/9783 (2017.08) [A61K 9/0014 (2013.01); A61K 36/738 (2013.01); A61Q 19/08 (2013.01)]

20 Claims

1. A meristematic cell line derived from a plant belonging to the genus Rosa, wherein said cell line is obtained by means of a process comprising the steps of:

1) plating a tissue obtained from a plant of the genus Rosa onto a solid culture medium;

2) isolating a plurality of cellular clones;

3) inoculating each of the isolated clones into a liquid culture medium;

4) determining the polysaccharide content for each clone;

5) selecting the cellular clone with the highest polysaccharide content,

wherein the solid and liquid culture media comprise salts suitable for the growth of plant cells, sucrose, naphthylacetic acid (NAA) and 6-benzyl amino purine (BAP) and

wherein the meristematic cell line comprises an amount of polysaccharides greater than 25% w/w.