	US 12,257,141 B2
	Preparation method for three-layer artificial blood vessel and application thereof
Chenxi Ouyang, Wuhan (CN); Jiarong Li, Wuhan (CN); Chenhong Wang, Wuhan (CN); Sishi Liu, Wuhan (CN); and Jianpeng Liu, Wuhan (CN)
Assigned to WUHAN YOUNGSEN BIOTECH CO., LTD., Wuhan (CN)
Filed by WUHAN YOUNGSEN BIOTECH CO., LTD., Wuhan (CN)
Filed on Jul. 16, 2021, as Appl. No. 17/377,427.
Application 17/377,427 is a continuation of application No. PCT/CN2020/072101, filed on Jan. 14, 2020.
Claims priority of application No. 201910040739.8 (CN), filed on Jan. 16, 2019; application No. 201910040748.7 (CN), filed on Jan. 16, 2019; and application No. 201910040750.4 (CN), filed on Jan. 16, 2019.
Prior Publication US 2021/0338410 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. D01D 1/02 (2006.01); A61F 2/00 (2006.01); A61F 2/06 (2013.01); B05D 1/00 (2006.01); D01D 5/00 (2006.01); D01D 10/02 (2006.01); D04H 1/728 (2012.01); B05D 1/02 (2006.01); B29C 48/151 (2019.01)

CPC A61F 2/06 (2013.01) [A61F 2/0077 (2013.01); A61F 2/062 (2013.01); B05D 1/002 (2013.01); D01D 5/003 (2013.01); D01D 5/0038 (2013.01); D01D 5/0061 (2013.01); D01D 5/0084 (2013.01); D04H 1/728 (2013.01); A61F 2002/0081 (2013.01); A61F 2210/0076 (2013.01); A61F 2240/001 (2013.01); A61F 2250/0058 (2013.01); A61L 2420/02 (2013.01); B05D 1/02 (2013.01); B05D 2254/02 (2013.01); B05D 2503/00 (2013.01); B05D 2518/00 (2013.01); B29C 48/151 (2019.02); D10B 2331/04 (2013.01); D10B 2331/041 (2013.01); D10B 2331/10 (2013.01); D10B 2509/00 (2013.01)]

7 Claims

1. A preparation method for three-layer artificial blood vessel, including the following steps:

S1: dissolving a macromolecular compound into a solvent to obtain an electrospinning solution with a concentration of 5-30% w/v and an electric-spray solution with a concentration of 10-60% w/v;

S2: placing the electrospinning solution obtained in S1 over a mandrel device and performing electrospinning to obtain a porous electrospun inner layer, drying the inner layer to remove the residual solvent;

S3: with the inner layer obtained in S2 as a receiving surface, performing first electric spray, second electric spray, . . . n^thelectric spray with the electric-spray solution to obtain a dense middle layer, wherein a process of the first electric spray includes spraying, stopping, spraying, stopping, . . . repeated cycles, a time of the stopping in the process of the first electric spray is 5-20 min; and a time of each of the first electric spray, the second electric spray and the n^thelectric spray is 5-50 min; and

S4: with the dense middle layer obtained in S3 as a receiving surface, performing electrospinning, and then drying to obtain the three-layer artificial blood vessel.