	US 12,256,758 B2
	Microbial-based process for high quality protein concentrate
William Gibbons, Brookings, SD (US); and Michael L Brown, Volga, SD (US)
Assigned to Prairie AquaTech LLC, Brookings, SD (US)
Filed by PRAIRIE AQUATECH LLC, Brookings, SD (US)
Filed on Sep. 12, 2022, as Appl. No. 17/942,759.
Application 17/942,759 is a continuation of application No. 17/243,315, filed on Apr. 28, 2021, granted, now 11,844,362.
Application 17/243,315 is a continuation of application No. 16/918,831, filed on Jul. 1, 2020, granted, now 11,147,292, issued on Oct. 19, 2021.
Application 16/918,831 is a continuation of application No. 15/151,599, filed on May 11, 2016, abandoned.
Application 15/151,599 is a continuation of application No. 13/691,843, filed on Dec. 2, 2012, granted, now 9,370,200, issued on Jun. 21, 2016.
Claims priority of provisional application 61/566,557, filed on Dec. 2, 2011.
Claims priority of provisional application 61/566,487, filed on Dec. 2, 2011.
Prior Publication US 2023/0093007 A1, Mar. 23, 2023
Int. Cl. A23J 1/12 (2006.01); A23J 1/14 (2006.01); A23K 10/14 (2016.01); A23K 10/38 (2016.01); A23K 20/147 (2016.01); A23K 40/20 (2016.01); A23K 40/25 (2016.01); A23K 50/80 (2016.01)

CPC A23J 1/125 (2013.01) [A23J 1/14 (2013.01); A23J 1/148 (2013.01); A23K 10/14 (2016.05); A23K 10/38 (2016.05); A23K 20/147 (2016.05); A23K 40/20 (2016.05); A23K 40/25 (2016.05); A23K 50/80 (2016.05); Y02A 40/818 (2018.01)]

11 Claims

1. A method of producing a soy protein concentrate having a specific lipid: protein weight ratio at about 0.010:1 to about 0.03:1 comprising:

a. screw-pressing or extruding soybean feedstock at above room temperature to form a mash and transferring the mash to a bioreactor;

b. optionally, adding one or more cellulose-degrading enzymes to release sugars into the mash in the bioreactor;

c. inoculating the enzyme treated or untreated mash with at least one microbes;

d. recovering proteins, cell mass from the at least one microbes, and exopolysaccharides from the incubated mash via filtration;

e. drying said recovered material,

wherein said soy protein concentrate comprises the proteins in an amount of at least about 70% by weight, the cell mass from the at least one microbes, and the exopolysaccharides, and wherein the protein content of the recovered material is greater than the protein content of the soybean feedstock of step (a).