	US 12,256,742 B2
	Compositions comprising recombinant bacillus cells and another biological control agent
Damian Curtis, Davis, CA (US); and Brian Thompson, Creve Coeur, MO (US)
Assigned to BASF CORPORATION, Florham Park, NJ (US)
Filed by BASF CORPORATION, Florham Park, NJ (US)
Filed on Oct. 16, 2023, as Appl. No. 18/487,437.
Application 18/487,437 is a continuation of application No. 17/813,065, filed on Jul. 18, 2022, granted, now 11,785,949.
Application 17/813,065 is a continuation of application No. 16/857,448, filed on Apr. 24, 2020, granted, now 11,388,903, issued on Jul. 19, 2022.
Application 16/857,448 is a continuation of application No. 15/511,822, granted, now 10,667,522, issued on Jun. 2, 2020, previously published as PCT/US2015/050592, filed on Sep. 17, 2015.
Claims priority of provisional application 62/051,911, filed on Sep. 17, 2014.
Prior Publication US 2024/0188570 A1, Jun. 13, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/002 (2006.01); A01N 37/46 (2006.01); A01N 63/22 (2020.01); A01N 63/23 (2020.01); A01N 63/50 (2020.01); C07K 14/32 (2006.01); A01N 37/00 (2006.01); C07K 14/00 (2006.01)

CPC A01N 63/50 (2020.01) [A01N 37/46 (2013.01); A01N 63/22 (2020.01); A01N 63/23 (2020.01); C07K 14/32 (2013.01); A01N 37/00 (2013.01); C07K 14/00 (2013.01)]

20 Claims

1. A method of treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health comprising the step of simultaneously or sequentially applying:

a) recombinant cells of a Bacillus cereus family member, wherein the cells express a fusion protein comprising:

(i) at least one plant growth stimulating protein or peptide selected from the group consisting of a polypeptide having endoglucanase activity and at least 90% sequence identity to SEQ ID NO: 107 and a phospholipase polypeptide having phospholipase activity and at least 90% sequence identity to SEQ ID NO: 108; and

(ii) a targeting sequence, exosporium protein, or exosporium protein fragment that is capable of targeting the fusion protein to the exosporium of the recombinant Bacillus cereus family member, comprising:

an amino acid sequence having at least 43% identity with amino acids 20-35 of SEQ ID NO: 1, wherein the identity with amino acids 25-35 is at least 54%;

amino acids 1-35 of SEQ ID NO: 1;

amino acids 20-35 of SEQ ID NO: 1;

amino acids 22-31 of SEQ ID NO: 1;

amino acids 22-33 of SEQ ID NO: 1;

amino acids 20-31 of SEQ ID NO: 1;

the amino acid sequence of SEQ ID NO: 1; or

an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 2; and

b) a Bacillus firmus strain

in a synergistic weight ratio in the range of 1:1000 to 1000:1, wherein the synergism refers to the effect of enhancing plant growth and/or promoting plant health.