US 12,256,737 B2
Preparation method and application of water-soluble chlorhexidine hyaluronate antibacterial agents
Kai Gong, Wuxi (CN); Xiuyun Han, Binzhou (CN); Haoran Li, Wuxi (CN); Ying Dong, Wuxi (CN); Huimin Zhang, Wuxi (CN); Huiru Feng, Wuxi (CN); Yanqin Liu, Wuxi (CN); and Xiangxiang Yu, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN); and Shandong AMHWA BIOPHARM CO., LTD., Binzhou (CN)
Filed on May 13, 2024, as Appl. No. 18/662,301.
Application 18/662,301 is a continuation of application No. PCT/CN2023/079839, filed on Mar. 6, 2023.
Claims priority of application No. 202210008312.1 (CN), filed on Jan. 6, 2022.
Prior Publication US 2024/0292841 A1, Sep. 5, 2024
Int. Cl. A01N 47/44 (2006.01); A01N 25/02 (2006.01)
CPC A01N 47/44 (2013.01) [A01N 25/02 (2013.01)] 6 Claims
 
1. A method for preparing a water-soluble chlorhexidine antimicrobial agent, comprising:
(a) adding while heating and stirring, a chlorhexidine solution dropwise into a hyaluronic acid solution to perform a complexation reaction therebetween thereby producing a reaction solution, wherein the hyaluronic acid solution comprises a first solvent selected from any one of water, formamide, DMSO, and DMF, and wherein the chlorhexidine solution comprises a second solvent selected from any one of formamide, DMSO, and DMF, and
(b) separating a product from the reaction solution obtained in step (a), and drying the product to a solid form to obtain a water-soluble chlorhexidine antimicrobial agent, wherein the drying comprises freeze-drying or spray-drying, and wherein the agent is hyaluronic acid chlorhexidine.