US 12,256,719 B2
Recombinant constructs and transgenic fluorescent ornamental fish therefrom
Alan Blake, Austin, TX (US); Richard Crockett, Wilton, CT (US); Jeffrey Essner, Ames, IA (US); Perry Hackett, Saint Paul, MN (US); and Aidas Nasevicius, Tampa, FL (US)
Assigned to GloFish LLC, Earth City, MO (US)
Filed by GloFish LLC, Earth City, MO (US)
Filed on May 12, 2023, as Appl. No. 18/316,688.
Application 18/316,688 is a continuation of application No. 16/915,859, filed on Jun. 29, 2020, granted, now 11,684,049.
Application 16/915,859 is a continuation of application No. 15/675,003, filed on Aug. 11, 2017, granted, now 10,743,526, issued on Aug. 18, 2020.
Application 15/675,003 is a continuation of application No. 15/041,253, filed on Feb. 11, 2016, granted, now 9,763,432, issued on Sep. 19, 2017.
Application 15/041,253 is a continuation of application No. 14/057,333, filed on Oct. 18, 2013, granted, now 9,271,478, issued on Mar. 1, 2016.
Application 14/057,333 is a continuation of application No. 13/663,210, filed on Oct. 29, 2012, granted, now 8,581,025, issued on Nov. 12, 2013.
Application 13/663,210 is a continuation of application No. 12/577,467, filed on Oct. 12, 2009, granted, now 9,295,237, issued on Mar. 29, 2016.
Application 12/577,467 is a continuation of application No. 11/839,364, filed on Aug. 15, 2007, granted, now 7,700,825, issued on Apr. 20, 2010.
Claims priority of provisional application 60/842,721, filed on Sep. 7, 2006.
Claims priority of provisional application 60/838,006, filed on Aug. 16, 2006.
Prior Publication US 2024/0138383 A1, May 2, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2024.01); A01K 67/0275 (2024.01); C07K 14/435 (2006.01); C12N 15/85 (2006.01); G06Q 99/00 (2006.01)
CPC A01K 67/0275 (2013.01) [A01K 67/027 (2013.01); C07K 14/43595 (2013.01); C12N 15/8509 (2013.01); G06Q 99/00 (2013.01); A01K 2217/05 (2013.01); A01K 2217/052 (2013.01); A01K 2217/206 (2013.01); A01K 2227/40 (2013.01); A01K 2267/0393 (2013.01); C12N 2830/008 (2013.01)] 17 Claims
 
1. A method of producing a transgenic, fluorescent fish having improved fluorescence comprising:
(a) obtaining transgenic, fluorescent fish that exhibit a fluorescence by virtue of their comprising:
i) a first transgene encoding a fluorescent protein, wherein the first transgene is under the control of a ubiquitous fish promoter, and
ii) a second transgene encoding the same fluorescent protein as in the first transgene, wherein the second transgene is under the control of a muscle-specific fish promoter, wherein said transgenic, fluorescent fish were generated by chromosomal introduction of said first and second transgenes positioned on a single vector; and
(b) selecting a transgenic fish exhibiting strong visible fluorescence over 75% to 100% of the body of said fish, optionally excluding fins and eyes and
(c) obtaining transgenic progeny of said selected transgenic fish, wherein the progeny exhibits fluorescence.