US 11,933,672 B2
Fabrication of gold nanoporous anodic aluminum oxide substrate of at-home blood phenylalanine measuring device for phenylketonuria
Richard D. Shunnarah, Atlanta, GA (US); Hugh F. Garvey, Seminole, FL (US); Lenzi J. Williams, Clearwater, FL (US); and Devon Fish, Largo, FL (US)
Assigned to METGEN, INCORPORATED, Atlanta, GA (US)
Filed by MetGen, Incorporated, Atlanta, GA (US)
Filed on Jul. 6, 2023, as Appl. No. 18/218,669.
Application 18/218,669 is a continuation of application No. 17/888,621, filed on Aug. 16, 2022, granted, now 11,747,199.
Claims priority of provisional application 63/234,771, filed on Aug. 19, 2021.
Prior Publication US 2023/0349756 A1, Nov. 2, 2023
Int. Cl. G01J 3/02 (2006.01); G01J 3/44 (2006.01); G01N 21/65 (2006.01); G01N 33/52 (2006.01); G01N 33/68 (2006.01); G01N 33/50 (2006.01)
CPC G01J 3/0272 (2013.01) [G01J 3/0256 (2013.01); G01J 3/4412 (2013.01); G01N 21/658 (2013.01); G01N 33/523 (2013.01); G01N 33/6812 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for fabrication of a gold nanoporous anodic aluminum oxide (NAAO) substrate used in coordination with a handheld Surface-Enhanced Raman Spectroscopy (SERS) device for detecting phenylalanine (Phe) in a sample collected from a subject, the method comprising:
forming a three-phase system on top of a multilayered nanoporous aluminum layer of the gold NAAO substrate; wherein the three-phase system comprises an air phase, a water phase, and an oil phase; and
injecting a cosolvent solution into the three-phase system, wherein the cosolvent solution comprises gold nanoparticles; and
drying the gold NAAO substrate by evaporating the water phase and the oil phase;
wherein the gold NAAO substrate formed is used in coordination with the handheld SERS device.