	US 11,932,897 B2
	Biosynthesis of curcuminoids in mammalian cells
Logan W. Warriner, Lexington, KY (US); and Daniel W. Pack, Lexington, KY (US)
Assigned to University of Kentucky Research Foundation, Lexington, KY (US)
Filed by University of Kentucky Research Foundation, Lexington, KY (US)
Filed on Dec. 18, 2019, as Appl. No. 16/719,843.
Claims priority of provisional application 62/781,343, filed on Dec. 18, 2018.
Prior Publication US 2020/0199630 A1, Jun. 25, 2020
Int. Cl. C12P 7/26 (2006.01); A61K 35/22 (2015.01); A61K 48/00 (2006.01); C12N 5/071 (2010.01); C12N 5/10 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/85 (2006.01)

CPC C12P 7/26 (2013.01) [A61K 35/22 (2013.01); C12N 5/0686 (2013.01); C12N 5/10 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/85 (2013.01); A61K 48/00 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01); C12N 2800/80 (2013.01); C12Y 114/14 (2013.01); C12Y 201/01104 (2013.01); C12Y 203/01 (2013.01); C12Y 403/01023 (2013.01); C12Y 602/01012 (2013.01); C12Y 604/01002 (2013.01)]

19 Claims

1. A method of making a curcuminoid in a mammalian cell, the method comprising:

expressing enzymes 4-coumaroyl-CoA ligase (4CL1) and one of 1) curcuminoid synthase (CUS) and 2) curcumin synthase (CURS1) and diketide-CoA synthase (DCS) in the mammalian cell, and optionally further expressing in the mammalian cell one or more additional enzymes selected from the group consisting of tyrosine ammonia lyase (TAL), DCS, CURS1, 4 coumarate 3- hydroxylase (C3H), caffeoyl-CoA 3-O-methyltransferase (CCoAOMT), and acetyl-CoA carboxylase (ACC); and

contacting the mammalian cell expressing the enzymes with a starting material being tyrosine, ferulic acid or p-coumaric acid to convert the starting material to the curcuminoid.