US 11,932,887 B2
CYP76AD1-beta clade polynucleotides, polypeptides, and uses thereof
Asaph Aharoni, Tel Aviv (IL); and Guy Polturak, Rehovot, IL (US)
Assigned to YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
Filed by YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
Filed on Jul. 28, 2020, as Appl. No. 16/940,666.
Application 16/940,666 is a continuation of application No. 15/758,372, granted, now 10,767,201, previously published as PCT/IL2016/051010, filed on Sep. 11, 2016.
Claims priority of application No. 241462 (IL), filed on Sep. 10, 2015.
Prior Publication US 2020/0354759 A1, Nov. 12, 2020
Int. Cl. C12P 13/22 (2006.01); A23L 3/3463 (2006.01); A23L 3/3526 (2006.01); A23L 3/3544 (2006.01); A23L 5/46 (2016.01); C07K 14/415 (2006.01); C12N 9/02 (2006.01); C12N 9/12 (2006.01); C12P 13/04 (2006.01); C12P 17/12 (2006.01); C12P 19/02 (2006.01)
CPC C12P 13/225 (2013.01) [A23L 3/3463 (2013.01); A23L 3/3526 (2013.01); A23L 3/3544 (2013.01); A23L 5/46 (2016.08); C07K 14/415 (2013.01); C12N 9/0083 (2013.01); C12N 9/1205 (2013.01); C12P 13/04 (2013.01); C12P 17/12 (2013.01); C12P 19/02 (2013.01); C12Y 207/01095 (2013.01); A23V 2002/00 (2013.01)] 8 Claims
 
1. A method of producing L-DOPA from tyrosine, comprising: introducing into a cell a recombinant polynucleotide that encodes CYP76AD6, wherein said recombinant polynucleotide is under the control of a heterologous promoter active in said cell, wherein said CYP76AD6 is a tyrosine hydroxylase that converts tyrosine to L-DOPA and comprises the amino acid sequence set forth in SEQ ID NO: 18 and wherein said cell is selected from a plant cell, a bacterial cell and a yeast cell, thereby producing L-DOPA from tyrosine.