US 11,932,884 B2
High efficiency base editors comprising Gam
David R. Liu, Cambridge, MA (US); Kevin Tianmeng Zhao, Cambridge, MA (US); and Yongjoo Kim, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Mar. 21, 2022, as Appl. No. 17/700,109.
Application 17/700,109 is a division of application No. 16/643,376, granted, now 11,319,532, previously published as PCT/US2018/048969, filed on Aug. 30, 2018.
Claims priority of provisional application 62/661,974, filed on Apr. 24, 2018.
Claims priority of provisional application 62/551,938, filed on Aug. 30, 2017.
Prior Publication US 2022/0290115 A1, Sep. 15, 2022
Int. Cl. C12N 9/78 (2006.01); C12N 9/22 (2006.01); C12N 15/62 (2006.01)
CPC C12N 9/78 (2013.01) [C12N 9/22 (2013.01); C12N 15/62 (2013.01); C07K 2319/09 (2013.01); C07K 2319/80 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A polynucleotide encoding a fusion protein comprising: (i) a nucleic acid programmable DNA binding protein (napDNAbp) domain; (ii) a cytidine deaminase domain; and (iii) a Gam protein.