CPC C12N 9/1241 (2013.01) [C12Y 207/07049 (2013.01)] | 18 Claims |
1. An engineered reverse transcriptase comprising a combination of mutations in the amino acid sequence of SEQ ID NO: 18, wherein the combination of mutations comprises:
(a) E69K, L139P, D200N, E302R, T306K, W313F, T330P, L435K, P448A, D449G, N454K, D524N, L603W, E607K; and
(b) one or more of A32V, M39V, M66L, L72R, L139P, F155Y, D200C, D200E, E201Q, H204R, G248C, E286R, T287A, Y344L, I347L, W388R, R411F, K435G, H503V, D583N, H594K, H594Q, L603F, E607G, H634Y, G637R, H638G, D653H, and L671P.
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