US 11,932,882 B2
Reverse transcriptase variants
Sonya A. Clark, Pleasanton, CA (US); Derek Hunter Vallejo, Pleasanton, CA (US); Carolina Cuellar, Pleasanton, CA (US); and Katherine Pfeiffer, Pleasanton, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Jun. 10, 2022, as Appl. No. 17/837,497.
Application 17/837,497 is a continuation of application No. PCT/US2020/064323, filed on Dec. 10, 2020.
Claims priority of provisional application 63/017,913, filed on Apr. 30, 2020.
Claims priority of provisional application 62/946,885, filed on Dec. 11, 2019.
Prior Publication US 2022/0403350 A1, Dec. 22, 2022
Int. Cl. C12N 9/12 (2006.01)
CPC C12N 9/1241 (2013.01) [C12Y 207/07049 (2013.01)] 18 Claims
 
1. An engineered reverse transcriptase comprising a combination of mutations in the amino acid sequence of SEQ ID NO: 18, wherein the combination of mutations comprises:
(a) E69K, L139P, D200N, E302R, T306K, W313F, T330P, L435K, P448A, D449G, N454K, D524N, L603W, E607K; and
(b) one or more of A32V, M39V, M66L, L72R, L139P, F155Y, D200C, D200E, E201Q, H204R, G248C, E286R, T287A, Y344L, I347L, W388R, R411F, K435G, H503V, D583N, H594K, H594Q, L603F, E607G, H634Y, G637R, H638G, D653H, and L671P.