US 11,932,881 B2
Heparin skeleton synthase and its mutants and application
Juzheng Sheng, Jinan (CN); Xueping Guo, Jinan (CN); Jianqun Deng, Jinan (CN); Fengshan Wang, Jinan (CN); Zhen Lu, Jinan (CN); Ranran Du, Jinan (CN); Liu Sun, Jinan (CN); and Yuanjun Sun, Jinan (CN)
Assigned to SHAN DONG UNIVERSITY, Jinan (CN); and Bloomage Biotechnology Corporation Limited, Jinan (CN)
Filed by SHAN DONG UNIVERSITY, Jinan (CN); and Bloomage Biotechnology Corporation Limited, Jinan (CN)
Filed on Jan. 3, 2022, as Appl. No. 17/646,773.
Claims priority of application No. 202110192424.2 (CN), filed on Feb. 20, 2021.
Prior Publication US 2022/0267746 A1, Aug. 25, 2022
Int. Cl. C12N 9/00 (2006.01); C12N 9/10 (2006.01); C12N 15/10 (2006.01); C12N 15/70 (2006.01); C12P 19/64 (2006.01)
CPC C12N 9/1051 (2013.01) [C12N 15/102 (2013.01); C12N 15/70 (2013.01); C12P 19/64 (2013.01); C12N 2800/101 (2013.01)] 7 Claims
 
1. A mutant heparin skeleton synthase (NaGlcNAc-T) is obtained from a wild-type NaGlcNAc-T, wherein the mutant NaGlcNAc-T is selected from the group consisting of a mutant heparin skeleton synthase having C16L mutation and comprises the amino acid sequence of SEQ ID NO: 4, a mutant heparin skeleton synthase having N25P mutation and comprises the amino acid sequence of SEQ ID NO: 6, a mutant heparin skeleton synthase having I30L mutation and comprises the amino acid sequence of SEQ ID NO: 8, a mutant heparin skeleton synthase having I111S mutation and comprises the amino acid sequence of SEQ ID NO: 10, a mutant heparin skeleton synthase having S165K mutation and comprises the amino acid sequence of SEQ ID NO: 12, a mutant heparin skeleton synthase having S172A mutation and comprises the amino acid sequence of SEQ ID NO: 14.