CPC C12N 9/1051 (2013.01) [C12N 15/102 (2013.01); C12N 15/70 (2013.01); C12P 19/64 (2013.01); C12N 2800/101 (2013.01)] | 7 Claims |
1. A mutant heparin skeleton synthase (NaGlcNAc-T) is obtained from a wild-type NaGlcNAc-T, wherein the mutant NaGlcNAc-T is selected from the group consisting of a mutant heparin skeleton synthase having C16L mutation and comprises the amino acid sequence of SEQ ID NO: 4, a mutant heparin skeleton synthase having N25P mutation and comprises the amino acid sequence of SEQ ID NO: 6, a mutant heparin skeleton synthase having I30L mutation and comprises the amino acid sequence of SEQ ID NO: 8, a mutant heparin skeleton synthase having I111S mutation and comprises the amino acid sequence of SEQ ID NO: 10, a mutant heparin skeleton synthase having S165K mutation and comprises the amino acid sequence of SEQ ID NO: 12, a mutant heparin skeleton synthase having S172A mutation and comprises the amino acid sequence of SEQ ID NO: 14.
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