	US 11,932,876 B2
	Stable three-dimensional blood vessels and methods for forming the same
Shahin Rafii, Ithaca, NY (US); and Brisa Palikuqi, Ithaca, NY (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Appl. No. 16/483,095
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
PCT Filed Feb. 2, 2018, PCT No. PCT/US2018/016640 § 371(c)(1), (2) Date Aug. 2, 2019, PCT Pub. No. WO2018/144860, PCT Pub. Date Aug. 9, 2018.
Claims priority of provisional application 62/454,161, filed on Feb. 3, 2017.
Prior Publication US 2019/0376044 A1, Dec. 12, 2019
Int. Cl. C12N 5/071 (2010.01); A61K 35/44 (2015.01); A61L 27/38 (2006.01); A61L 27/50 (2006.01)

CPC C12N 5/0691 (2013.01) [A61K 35/44 (2013.01); A61L 27/3808 (2013.01); A61L 27/507 (2013.01); A61L 2430/22 (2013.01); A61L 2430/40 (2013.01); C12N 2500/02 (2013.01); C12N 2510/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01)]

22 Claims

1. A method for producing a three-dimensional blood vessel comprising:

a) culturing an endothelial cell comprising an exogenous nucleic acid encoding an ETV2 transcription factor on a matrix under conditions wherein the endothelial cell expresses the ETV2 transcription factor for at least 3-4 weeks to induce formation of a three-dimensional blood vessel; and

b) isolating said three-dimensional blood vessel.