US 11,932,868 B2
Microplates for magnetic 3D culture
Glauco Souza, Houston, TX (US)
Assigned to GREINER BIO-ONE NORTH AMERICA
Filed by Greiner Bio-One North America, Inc., Monroe, NC (US)
Filed on Feb. 16, 2021, as Appl. No. 17/176,188.
Application 17/176,188 is a division of application No. 14/965,702, filed on Dec. 10, 2015, abandoned.
Application 14/965,702 is a continuation in part of application No. 14/331,377, filed on Jul. 15, 2014, granted, now 9,909,116, issued on Mar. 6, 2018.
Application 14/331,377 is a continuation in part of application No. 13/762,103, filed on Feb. 7, 2013, granted, now 10,407,660, issued on Sep. 10, 2019.
Application 13/762,103 is a continuation of application No. PCT/US2011/046183, filed on Aug. 2, 2011.
Application 14/965,702 is a continuation in part of application No. 13/070,873, filed on Mar. 24, 2011, granted, now 8,815,231, issued on Aug. 26, 2014.
Application 13/070,873 is a continuation of application No. PCT/US2009/058473, filed on Sep. 29, 2009.
Claims priority of provisional application 61/372,164, filed on Aug. 10, 2010.
Claims priority of provisional application 61/099,966, filed on Sep. 25, 2008.
Prior Publication US 2021/0163881 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); B01L 3/00 (2006.01); C12M 1/22 (2006.01); C12M 1/26 (2006.01); C12M 1/32 (2006.01); C12M 1/42 (2006.01); C12N 5/00 (2006.01); C12N 13/00 (2006.01)
CPC C12N 5/0062 (2013.01) [B01L 3/50853 (2013.01); C12M 23/10 (2013.01); C12M 23/12 (2013.01); C12M 23/38 (2013.01); C12M 33/00 (2013.01); C12M 33/04 (2013.01); C12M 35/06 (2013.01); C12M 47/04 (2013.01); C12N 13/00 (2013.01); B01L 2300/046 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/043 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A magnetic culture plate for three-dimensional (3D) cell culturing, comprising:
a) a standard ANSI-SLAS microtiter plate comprising a plurality of wells in an array and having a length of 127.76 mm ±0.5 mm and a width of 85.48 mm ±0.5 mm,
b) a cap comprising a magnetic holder and an intervening cover, wherein said cap is sized to fit over and cover said standard ANSI-SLAS microtiter plate, said cap having a lip around a circumference thereof and a plurality of magnets in an array, each magnet affixed to a depression in said magnetic holder, such that adjacent magnets are oriented in opposite polarity, wherein each magnet of the plurality of magnets protrudes inside a corresponding well of said intervening cover, said intervening cover being arranged between the magnets and the standard ANSI-SLAS microtiter plate and having a number of wells corresponding to a number of magnets of the plurality of magnets, said wells of the intervening cover configured to receive the magnets and to protrude inside the wells of the standard ANSI-SLAS microtiter plate when said cap is fitted in place over said standard ANSI-SLAS microtiter plate, such that each well of the standard ANSI-SLAS microtiter plate has a single magnet protruding inside said well of the standard ANSI-SLAS microtiter plate when said cap is fitted in place over said standard ANSI-SLAS microtiter plate, wherein the magnets do not touch a culture media inside said well, and wherein the cap holds the magnets at a fixed distance of 0.2 to 10 mm from said culture media inside said well of the standard ANSI-SLAS microtiter plate.