US 11,932,861 B2
Virus-based replicon for plant genome editing without inserting replicon into plant genome and uses thereof
Jae Yean Kim, Gyeongsangnam-do (KR); Tien Van Vu, Gyeongsangnam-do (KR); Jihae Kim, Gyeongsangnam-do (KR); Se Jeong Jeong, Gyeongsangnam-do (KR); Hyun Jeong Kim, Gyeongsangnam-do (KR); Seo-Jin Park, Gyeongsangnam-do (KR); Mil Thi Tran, Gyeongsangnam-do (KR); Velu Sivankalyani, Gyeongsangnam-do (KR); Yeon Woo Sung, Gyeongsangnam-do (KR); Thi Hai Duong Doan, Gyeongsangnam-do (KR); Dibyajyoti Pramanik, Gyeongsangnam-do (KR); Mahadev Rahul Shelake, Gyeongsangnam-do (KR); and Geon Hui Son, Gyeongsangnam-do (KR)
Assigned to INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY, Gyeongsangnam-Do (KR)
Appl. No. 17/274,559
Filed by INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY, Gyeongsangnam-do (KR)
PCT Filed Sep. 10, 2019, PCT No. PCT/KR2019/011677
§ 371(c)(1), (2) Date Mar. 9, 2021,
PCT Pub. No. WO2020/055084, PCT Pub. Date Mar. 19, 2020.
Claims priority of application No. 10-2018-0108026 (KR), filed on Sep. 11, 2018.
Prior Publication US 2022/0049263 A1, Feb. 17, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8203 (2013.01) [C12N 2750/12043 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A recombinant vector for genome editing without inserting a replicon into plant genome in a T0 generation plant, the recombinant vector including a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid,
wherein the geminivirus is BeYDV (Bean Yellow Dwarf Virus),
wherein the replicon has a first long intergenic region (LIR), a Rep/RepA protein-coding sequence, a short intergenic region (SIR), a CaMV 35S terminator, a Cpf1 coding sequence, a CaMV 35s promoter having AtUBQ intron, U6-double gRNAs, a first template DNA site for gene-editing, a NOS promoter, NPTII coding sequence, a OCS (octopine synthase) terminator, a CaMV 35S promoter, a second template DNA site for gene-editing, and a second long intergenic region (LIR) that are sequentially connected.