CPC C12N 15/1055 (2013.01) [C12N 15/03 (2013.01); C12Q 1/025 (2013.01); C12Q 1/48 (2013.01); C12Y 203/01028 (2013.01); G01N 2333/91062 (2013.01); G01N 2500/02 (2013.01)] | 14 Claims |
1. A construct system comprising:
(i) a first nucleic acid construct comprising a first polynucleotide having a nucleic acid sequence that encodes a N-terminal fragment of chloramphenicol acetyl transferase (CAT), said N-terminal fragment comprising a first portion of the catalytic active site of said CAT, said N-terminal fragment being devoid of acetylating activity; and
(ii) a second nucleic acid construct comprising a second polynucleotide having a nucleic acid sequence that encodes a C terminal fragment of said CAT, said C-terminal fragment comprising a second portion of the catalytic active site of said CAT, said C-terminal fragment being devoid of acetylating activity; and
wherein said N-terminal fragment is capable of associating with said C-terminal fragment to generate an active CAT that is capable of acetylating chloramphenicol,
wherein said N terminal fragment consists of the amino acid sequence as set forth in SEQ ID NO: 2 or 6; and/or
said C-terminal fragment consists of the amino acid sequence as set forth in SEQ ID NOs: 3 or 7.
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