US 11,932,836 B2
Electric reaction measuring apparatus, electric reaction processing method, and recording medium
Yumiko Kato, Osaka (JP); and Kiyotaka Tsuji, Osaka (JP)
Assigned to PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD., Osaka (JP)
Filed by Panasonic Intellectual Property Management Co., Ltd., Osaka (JP)
Filed on Jul. 2, 2020, as Appl. No. 16/920,356.
Application 16/920,356 is a continuation of application No. PCT/JP2019/006439, filed on Feb. 21, 2019.
Claims priority of application No. 2018-046060 (JP), filed on Mar. 13, 2018.
Prior Publication US 2020/0333285 A1, Oct. 22, 2020
Int. Cl. C12M 1/34 (2006.01); C12M 1/32 (2006.01)
CPC C12M 1/3407 (2013.01) [C12M 23/12 (2013.01); C12M 41/46 (2013.01)] 9 Claims
 
1. An apparatus comprising:
a culture vessel, wherein the culture vessel includes a partition member disposed on a bottom wall of the culture vessel, wherein the partition member includes one or more chambers, wherein one or more electrodes are disposed in the one or more chambers, wherein each of the one or more electrodes are composed of at least one reference electrode and at least one measurement electrode that are disposed about a partition wall that separates the one or more chambers from each other;
a memory storing a program; and
a processor configured to execute the program to:
measure, for each of the one or more electrodes, a potential of the at least one measurement electrode of the one or more electrodes at one or more predetermined times;
determine at least one principal component for each of the at least one measurement electrode of the one or more electrodes by performing principal component analysis of the potential of each of the at least one measurement electrode of the one or more electrodes at the one or more predetermined times;
estimate, for each of the at least one measurement electrode of the one or more electrodes, a principal component potential due to the principal component from the principal component of the at least one measurement electrode of the one or more electrodes;
synthesize the estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes;
subtract the synthesized estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes from the potential measured at the at least one measurement electrode of the one or more electrodes so as to remove noise; and
output a potential difference based on the subtraction that reflects an activity of an object in the culture vessel.