CPC C07K 16/303 (2013.01) [A61P 35/00 (2018.01); C07K 16/2809 (2013.01); A61K 2039/505 (2013.01); C07K 2317/31 (2013.01); C07K 2317/33 (2013.01); C07K 2317/565 (2013.01); C07K 2317/569 (2013.01); C07K 2317/73 (2013.01); C07K 2317/92 (2013.01); C07K 2319/30 (2013.01)] | 28 Claims |
1. A polypeptide that comprises at least three immunoglobulin single variable domains (ISVDs), wherein each of said ISVDs comprises three complementarity determining regions (CDRs), CDR1 to CDR3, respectively, and wherein:
a) a first ISVD specifically binds to the constant domain of a T cell receptor (TCR) on a T cell and comprises:
i. a CDR1 comprising the amino acid sequence of SEQ ID NO: 6;
ii. a CDR2 comprising the amino acid sequence of SEQ ID NO: 10; and
iii. a CDR3 comprising the amino acid sequence of SEQ ID NO: 14;
b) a second ISVD specifically binds to Glypican-3 (GPC3) and comprises:
iv. a CDR1 comprising the amino acid sequence of SEQ ID NO: 7;
v. a CDR2 comprising the amino acid sequence of SEQ ID NO: 11; and
vi. a CDR3 comprising the amino acid sequence of SEQ ID NO: 15; and
c) a third ISVD specifically binds to GPC3 and comprises:
vii. a CDR1 comprising the amino acid sequence of SEQ ID NO: 8;
viii. a CDR2 comprising the amino acid sequence of SEQ ID NO: 12; and
ix. a CDR3 comprising the amino acid sequence of SEQ ID NO: 16,
wherein the order of the ISVDs indicates their relative position to each other considered from the N-terminus to the C-terminus of said polypeptide.
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