US 11,932,672 B2
Fermentation process
Philippe Gabant, Ottignies Louvain-la-Neuve (BE); Mohamed El Bakkoury, Brussels (BE); and Laurence Van Melderen, Waterloo (BE)
Assigned to Syngulon S.A., Seraing (BE); and Université Libre de Bruxelles, Brussels (BE)
Appl. No. 16/955,720
Filed by Syngulon S.A., Seraing (BE); and Université Libre de Bruxelles, Brussels (BE)
PCT Filed Dec. 19, 2018, PCT No. PCT/EP2018/085941
§ 371(c)(1), (2) Date Jun. 18, 2020,
PCT Pub. No. WO2019/121983, PCT Pub. Date Jun. 27, 2019.
Claims priority of application No. 17208600 (EP), filed on Dec. 19, 2017.
Prior Publication US 2021/0070812 A1, Mar. 11, 2021
Int. Cl. C07K 14/245 (2006.01); C12N 15/113 (2010.01); C12N 15/70 (2006.01)
CPC C07K 14/245 (2013.01) [C12N 15/113 (2013.01); C12N 15/70 (2013.01); C12N 2800/101 (2013.01); C12N 2800/204 (2013.01)] 17 Claims
 
1. A method for producing a product of interest with a microbial host, said method comprising the steps of:
a) providing the microbial host comprising i) an auto-replicative extra-chromosomal nucleic acid molecule comprising a first nucleic acid sequence whose genetic activity confers immunity or resistance to a bacteriocin to the microbial host, wherein the genetic activity of said first nucleic acid sequence is controlled and ii) a second nucleic acid sequence coding for said product of interest; and
b) culturing said transformed microbial host under conditions allowing said transformed microbial host to express the first nucleic acid sequence to a given level such that the genetic activity of the first nucleic acid sequence confers a selective advantage to the microbial host during the culturing, to thereby maintain the auto-replicative extra-chromosomal molecule in the growing microbial population, and simultaneously genetically controlling the second nucleic acid sequence to produce said product of interest,
wherein during at least a portion of the culturing of step b) conditions are such that the first nucleic acid sequence does not exhibit said genetic activity thereby reducing the energetic burden for the microbial host cell during the production of the product of interest.