US 11,931,737 B2
Platforms and systems for automated cell culture
Matthias Wagner, Cambridge, MA (US); Suvi Aivio, Arlington, MA (US); Mariangela Amenduni, Arlington, MA (US); Catherine Pilsmaker, Arlington, MA (US); Arnaldo Pereira, Cambridge, MA (US); Ananya Zutshi, Boston, MA (US); Ozge Whiting, Pawtucket, RI (US); George Harb, Providence, RI (US); Steven Nagle, Wayland, MA (US); Anthia Toure, Boston, MA (US); Matthew Sullivan, Westwood, MA (US); Maya Berlin-Udi, Acton, MA (US); Lukas Vasadi, Cambridge, MA (US); Alexander Stange, Somerville, MA (US); Sangkyun Lee, Newton, MA (US); Stefanie Morgan, Hanover, MA (US); Nick Seay, Charlottesville, VA (US); and Scott Luro, Somerville, MA (US)
Assigned to CELLINO BIOTECH, INC., Cambridge, MA (US)
Filed by Cellino Biotech, Inc., Cambridge, MA (US)
Filed on Sep. 7, 2022, as Appl. No. 17/930,413.
Claims priority of provisional application 63/370,994, filed on Aug. 10, 2022.
Claims priority of provisional application 63/370,912, filed on Aug. 9, 2022.
Claims priority of provisional application 63/370,486, filed on Aug. 4, 2022.
Claims priority of provisional application 63/370,209, filed on Aug. 2, 2022.
Claims priority of provisional application 63/369,900, filed on Jul. 29, 2022.
Claims priority of provisional application 63/369,589, filed on Jul. 27, 2022.
Claims priority of provisional application 63/391,453, filed on Jul. 22, 2022.
Claims priority of provisional application 63/347,506, filed on May 31, 2022.
Claims priority of provisional application 63/311,673, filed on Feb. 18, 2022.
Claims priority of provisional application 63/298,241, filed on Jan. 11, 2022.
Claims priority of provisional application 63/297,290, filed on Jan. 7, 2022.
Claims priority of provisional application 63/295,968, filed on Jan. 3, 2022.
Claims priority of provisional application 63/288,859, filed on Dec. 13, 2021.
Claims priority of provisional application 63/284,839, filed on Dec. 1, 2021.
Claims priority of provisional application 63/282,351, filed on Nov. 23, 2021.
Claims priority of provisional application 63/249,698, filed on Sep. 29, 2021.
Claims priority of provisional application 63/239,995, filed on Sep. 2, 2021.
Prior Publication US 2023/0065504 A1, Mar. 2, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 3/00 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12N 5/00 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2006.01); G06T 7/00 (2017.01)
CPC B01L 3/502715 (2013.01) [C12M 23/14 (2013.01); C12M 23/22 (2013.01); C12M 23/26 (2013.01); C12M 23/42 (2013.01); C12M 23/44 (2013.01); C12M 23/48 (2013.01); C12M 25/06 (2013.01); C12M 27/02 (2013.01); C12M 29/02 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/48 (2013.01); C12M 47/04 (2013.01); C12N 5/0081 (2013.01); G01N 15/1056 (2013.01); G06T 7/0016 (2013.01); B01L 2300/0663 (2013.01); G01N 2015/1006 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A cell culture system, comprising:
(i) a cell culture container, comprising:
a cell culture chamber enclosing fluid media, wherein the cell culture chamber comprises a first surface interior to the cell culture chamber, wherein the first surface is at least partially transparent;
a plasmonic resonant film disposed on the first surface and permanently attached thereto, wherein the plasmonic resonant film is configured to at least partially absorb a first incident light within a first wavelength range and at least partially transmit a second incident light within a second wavelength range, and wherein the plasmonic resonant film exhibits a spectral response comprising a resonant absorption peak; and
a cell culture comprising a plurality of cell colonies adhered to the first surface; and
(ii) a cell editing subsystem comprising a laser configured to emit a laser pulse incident on the plasmonic resonant film, wherein the cell editing subsystem is configured to selectively destroy one or more cells of the cell culture, when the laser emits the laser pulse at the resonant absorption peak;
(iii) an imaging subsystem configured to generate one or more images of the cell culture; and
(iv) a computing subsystem configured to manage the cell culture in the cell culture container, wherein managing the cell culture comprises at least one of:
(a) analyzing the one or more images of the cell culture to predict a collision between a first cell colony and a second cell colony in the plurality of cell colonies, and removing the first cell colony using the cell editing subsystem thereby avoiding the predicted collision,
(b) analyzing the one or more images of the cell culture to determine that a cell colony in the plurality of cell colonies is approaching full confluency, and fragmenting the cell colony into a plurality of sub-colonies thereby reducing confluency of the cell colony, and
(c) analyzing the one or more images of the cell culture to determine that a cell colony in the plurality of cell colonies has excessive density, and removing a portion of the cell colony thereby reducing density of the cell colony.