US 11,931,719 B2
Chromatography media and methods for producing them
Marc Hummersone, Hertfordshire (GB); Benjamin Wallis, Hertfordshire (GB); Frederic Leroux, Hertfordshire (GB); and William Howard Albert Law, Hertfordshire (GB)
Assigned to PURIDIFY LTD., Hertfordshire (GB)
Appl. No. 16/959,373
Filed by Puridify Ltd., Hertfordshire (GB)
PCT Filed Jan. 7, 2019, PCT No. PCT/EP2019/050227
§ 371(c)(1), (2) Date Jun. 30, 2020,
PCT Pub. No. WO2019/137869, PCT Pub. Date Jul. 18, 2019.
Claims priority of application No. 1800448 (GB), filed on Jan. 11, 2018.
Prior Publication US 2021/0060527 A1, Mar. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01J 20/26 (2006.01); B01J 20/285 (2006.01); B01J 20/32 (2006.01); B01J 39/05 (2017.01); B01J 39/07 (2017.01); B01J 41/05 (2017.01); B01J 41/07 (2017.01)
CPC B01J 20/265 (2013.01) [B01D 15/327 (2013.01); B01D 15/362 (2013.01); B01D 15/363 (2013.01); B01J 20/285 (2013.01); B01J 20/3274 (2013.01); B01J 39/05 (2017.01); B01J 39/07 (2017.01); B01J 41/05 (2017.01); B01J 41/07 (2017.01); B01J 2219/00844 (2013.01); B01J 2219/00846 (2013.01)] 42 Claims
OG exemplary drawing
 
1. A functionalised chromatography medium, comprising:
i) at least one non-woven layer of polymeric nanofibres comprising a plurality of nanofibre-nanofibre fusion points;
ii) a grafted polymer coating covering said polymeric nanofibres and said nanofibre-nanofibre fusion points, wherein the grafted polymer coating comprises polymer molecules which are single-point covalently tethered to said polymeric nanofibres, and wherein the polymer molecules comprise glycidol monomer residues formed from a plurality of compounds of formula

OG Complex Work Unit Chemistry
and
iii) a plurality of ligand groups covalently bound to said grafted polymer coating, wherein said ligand groups are capable of interacting with a target biomolecule.