	US 11,931,713 B2
	Oligonucleotide data storage on solid supports
Jeffrey Kenneth Horton, Cardiff (GB); Peter James Tatnell, Cardiff (GB); and Robert Stone, Cardiff (GB)
Assigned to Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
Appl. No. 15/102,209
Filed by Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
PCT Filed Nov. 26, 2014, PCT No. PCT/EP2014/075708 § 371(c)(1), (2) Date Jun. 6, 2016, PCT Pub. No. WO2015/090879, PCT Pub. Date Jun. 25, 2015.
Claims priority of application No. 1322400 (GB), filed on Dec. 18, 2013.
Prior Publication US 2017/0151545 A1, Jun. 1, 2017
Int. Cl. B01J 19/00 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6874 (2018.01)

CPC B01J 19/0046 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/6874 (2013.01); B01J 2219/00529 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00722 (2013.01)]

21 Claims

1. A data storage medium comprising a solid support matrix adapted for use as a dry storage support for non-naturally occurring oligonucleotide sequences encoded with ternary or quaternary base system DNA data,

wherein the matrix is fibrous,

wherein said matrix includes a reagent mix in a dry form comprising a combination of a weak base, a chelating agent, a stabilising reagent, and an anionic surfactant,

wherein the stabilising reagent comprises a chaotropic salt, and

wherein the storage medium includes a visual delineation around an area for holding the DNA data comprising: (i) a printed boundary on the fibrous matrix configured to show where the DNA data is stored to facilitate sampling therefrom or (ii) an indicating dye of chlorophenol red or phenol red which is configured to discolour, thereby showing where the DNA data is stored on the storage medium to facilitate sampling therefrom.