CPC A61K 36/28 (2013.01) [A23L 2/52 (2013.01); A23L 33/105 (2016.08); A61K 31/216 (2013.01); A61K 36/185 (2013.01); B01D 15/125 (2013.01); B01D 15/161 (2013.01); B01D 15/363 (2013.01); B01D 15/426 (2013.01); A23V 2002/00 (2013.01); A61K 2236/31 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/37 (2013.01); A61K 2236/39 (2013.01); A61K 2236/55 (2013.01)] | 30 Claims |
1. A method for making a caffeoylquinic composition, the method comprising:
i) contacting verba mate biomass, stevia biomass, or combinations thereof with a first aqueous composition comprising less than 30% (C1-C4)alkanol or free of (C1-C4)alkanol to obtain an initial extract;
ii) chromatographing the initial extract on an ion exchange stationary phase;
iii) eluting the stationary phase with a first aqueous elution composition comprising 10%-50% (C1-C4)alkanol to obtain a first eluent; and
iv) eluting the stationary phase with an acidified second aqueous elution composition comprising 60% to 80% (C1-C4)alkanol to obtain the caffeoylquinic composition,
wherein the caffeoylquinic composition comprises monocaffeoylquinic acid, dicaffeoylquinic acid, and salts of the foregoing; and
wherein the composition comprises less than 1% caffeine based on dry weight of the caffeoylquinic composition.
|