| CPC G01Q 10/065 (2013.01) | 15 Claims |

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1. A method of examining a sample in an atomic force microscope including at least one probe, each probe including at least one cantilever and at least one tip, comprising
carrying out the following sequence of procedures:
a negative setpoint SCD setting procedure (a), wherein the negative setpoint SCD is set as a negative real number;
an approaching procedure (b) comprising: above at least one point of the sample, performing an approach comprising reducing the tip-to-sample distance D and recording cantilever deflection CD as a function of the tip-to-sample distance D, while applying the standard sign convention with cantilever deflection CD considered negative for attractive forces and positive for repulsive forces, until at least one of critical criteria is achieved, the critical criteria consisting of or comprising:
a first critical criterium (b1): the cantilever deflection CD reaches the value substantially equal to the negative setpoint SCD, i.e. CD≅SCD:
a second critical criterium (b2): the cantilever deflection CD reaches the value substantially equal to the negative setpoint SCD divided by two and then the derivative of cantilever deflection in time reaches the value substantially equal to zero, i.e. CD≅SCD/2 is reached and then
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a third critical criterium (b3): the cantilever deflection CD reaches the value substantially equal to the negative setpoint SCD divided by two and then the cantilever deflection CD reaches the value substantially equal to zero, i.e. CD≅SCD/2 is reached and then CD≅0 is reached;
a fourth critical criterium (b4): the cantilever deflection CD reaches the value substantially equal to minus negative setpoint SCD, i.e. CD≅−SCD, and
pausing or stopping of the approach after performing the approaching procedure.
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