US 12,253,530 B2
Diagnosis method of multiple sclerosis
Eric Thouvenot, Saint-Gely-du-Fesc (FR); Philippe Marin, Saint-Gely-du-Fesc (FR); Serge Urbach, Juvignac (FR); and Geoffrey Hinsinger, Montpellier (FR)
Assigned to CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICAL (INSERM), Paris (FR); UNIVERSITE DE MONTPELLIER, Montpellier (FR); and CENTRE HOSPITALIER UNIVERSITAIRE DE NÎMES, NÎmes (FR)
Appl. No. 17/057,269
Filed by CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICAL (INSERM), Paris (FR); UNIVERSITE DE MONTPELLIER, Montpellier (FR); and CENTRE HOSPITALIER UNIVERSITAIRE DE NÎMES, Nîmes (FR)
PCT Filed May 22, 2019, PCT No. PCT/EP2019/063208
§ 371(c)(1), (2) Date Nov. 20, 2020,
PCT Pub. No. WO2019/224242, PCT Pub. Date Nov. 28, 2019.
Claims priority of application No. 18305630 (EP), filed on May 22, 2018.
Prior Publication US 2021/0364533 A1, Nov. 25, 2021
Int. Cl. G01N 33/68 (2006.01)
CPC G01N 33/6896 (2013.01) [G01N 2333/4722 (2013.01); G01N 2333/70578 (2013.01); G01N 2333/82 (2013.01); G01N 2333/978 (2013.01); G01N 2800/285 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for diagnosing multiple sclerosis in an individual suspected to be afflicted by multiple sclerosis, comprising the steps of:
a) measuring, in a biological sample of said individual, the amount of at least one first protein, said at least one first protein comprising the amino acid sequence as set forth in SEQ ID NO: 1, said at least one first protein belonging to the group of proteins consisting of a first protein, a second protein, a third protein, a fourth protein and a fifth protein,
said first protein comprising the amino acid sequence as set forth in SEQ ID NO: 1,
said second protein comprising the amino acid sequence as set forth in SEQ ID NO: 2,
said third protein comprising the amino acid sequence as set forth in SEQ ID NO: 3,
said fourth protein comprising the amino acid sequence as set forth in SEQ ID NO: 4, and
said fifth protein comprising the amino acid sequence as set forth in SEQ ID NO: 5,
b) comparing the amount of said at least one first protein with the amount of the same protein in a control sample of the same nature originating from a healthy individual not afflicted by multiple sclerosis, to establish a protein expression level ratio of said at least one first protein,
c) diagnosing said individual as having multiple sclerosis for the ratio established in step b) for said at least one first protein is higher than, or equal to, 2.