US 12,253,521 B2
Early detection of colorectal cancer
Carole A. Oskeritzian, Columbia, SC (US); Franklin Berger, Columbia, SC (US); Alena P. Chumanevich, Lexington, SC (US); John W. Fuseler, Columbia, SC (US); Nabihah I. Kumte, Columbia, SC (US); and Ahmed Aladhami, Columbia, SC (US)
Assigned to UNIVERSITY OF SOUTH CAROLINA, Columbia, SC (US)
Filed by UNIVERSITY OF SOUTH CAROLINA, Columbia, SC (US)
Filed on Jun. 29, 2023, as Appl. No. 18/344,286.
Application 18/344,286 is a continuation of application No. 16/720,309, filed on Dec. 19, 2019, granted, now 11,714,088.
Claims priority of provisional application 62/807,403, filed on Feb. 19, 2019.
Prior Publication US 2023/0341402 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/574 (2006.01); A61B 10/00 (2006.01); A61B 10/02 (2006.01); G01N 1/30 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/57419 (2013.01) [A61B 10/0096 (2013.01); A61B 10/02 (2013.01); G01N 1/30 (2013.01); G01N 33/5091 (2013.01); G01N 2001/302 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for detection of colorectal cancer or precancerous colon polyps, the method comprising:
swabbing a rectal area of a subject and thereby collecting a cell sample on the swab;
sealing the cell sample in a container, the container carrying a buffer solution; and
detecting according to a computerized imaging approach the presence or quantity of sloughed colonic mast cells in the sample, wherein the computerized imaging approach comprises identifying areas of the stained cell sample that include slain-positive cells, and isolating individual regions of interest in the identified areas according to a Hue, Saturation, Intensity (HSI) color model;
wherein the presence of the sloughed colonic mast cells in the cell sample indicates the presence of colorectal cancer or precancerous polyps in the colon of the subject.