US 12,253,517 B2
Nano-enzyme containers for test elements
Grit Baier, Illertissen (DE); Carina Horn, Biblis (DE); Katharina Landfester, Mainz (DE); Anna Musyanovych, Mainz (DE); and Umaporn Paiphansiri, Ludwigshafen (DE)
Assigned to ROCHE DIABETES CARE, INC., Indianapolis, IN (US)
Filed by Roche Diabetes Care, Inc., Indianapolis, IN (US)
Filed on Aug. 20, 2021, as Appl. No. 17/407,283.
Application 17/407,283 is a division of application No. 15/141,062, filed on Apr. 28, 2016, granted, now 11,105,800.
Application 15/141,062 is a continuation of application No. PCT/EP2014/072994, filed on Oct. 27, 2014.
Claims priority of application No. 13190709 (EP), filed on Oct. 29, 2013.
Prior Publication US 2021/0382042 A1, Dec. 9, 2021
Int. Cl. G01N 33/543 (2006.01); C08G 18/28 (2006.01); C12N 9/04 (2006.01); C12N 11/093 (2020.01); C12N 11/098 (2020.01); C12Q 1/00 (2006.01); C12Q 1/32 (2006.01); C12Q 1/54 (2006.01); G01N 33/487 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/5432 (2013.01) [C08G 18/289 (2013.01); C12N 9/0006 (2013.01); C12N 11/093 (2020.01); C12N 11/098 (2020.01); C12Q 1/006 (2013.01); C12Q 1/32 (2013.01); C12Q 1/54 (2013.01); G01N 33/48721 (2013.01); G01N 33/5038 (2013.01); G01N 33/54346 (2013.01)] 12 Claims
 
1. A diagnostic test element for the detection of an analyte comprising:
an enzyme; and
a silica nanocapsule substantially impermeable for the enzyme but permeable for an enzyme substrate, the enzyme being incorporated within the interior of the silica nanocapsule, the interior of the silica nanocapsule being free from a hydrophilic polymer,
wherein the silica nanocapsule comprises a polyaddition and/or polycondensation product of monomers formed at the interface in a water-in-oil miniemulsion comprising a continuous oil phase and a discontinuous aqueous phase dispersed therein, the enzyme being present in the aqueous phase, and
wherein the silica nanocapsule is a polycondensation product of a tetra-alkoxy silane with a compound RSiX3,
wherein X is halo and
R is an optionally substituted C3-C30alkyl group.