	US 12,253,514 B2
	Compositions and methods for modulating kinase activity
Dorothee Kern, Waltham, MA (US); and Adelajda Zorba, Waltham, MA (US)
Assigned to Brandeis University, Waltham, MA (US)
Filed by Brandeis University, Waltham, MA (US)
Filed on Jul. 20, 2021, as Appl. No. 17/380,704.
Application 17/380,704 is a division of application No. 15/774,747, granted, now 11,104,741, previously published as PCT/US2016/015171, filed on Jan. 27, 2016.
Claims priority of provisional application 62/254,974, filed on Nov. 13, 2015.
Prior Publication US 2023/0050231 A1, Feb. 16, 2023
Int. Cl. G01N 33/50 (2006.01)

CPC G01N 33/5008 (2013.01) [G01N 2333/47 (2013.01); G01N 2333/91205 (2013.01); G01N 2500/04 (2013.01)]

10 Claims

1. A method of identifying an antibody mimetic that specifically binds to an allosteric site of Aurora A kinase, the method comprising

(a) contacting a candidate antibody mimetic with an Aurora A kinase having an unbound allosteric site,

(b) contacting the candidate antibody mimetic with an Aurora A kinase having a bound allosteric site or an Aurora A kinase mutant that has a mutation within an allosteric site,

(c) measuring binding of the candidate antibody mimetic with Aurora A kinase in step (a) and in step (b), wherein a candidate antibody mimetic having an increased binding to Aurora A kinase in step (a) relative to step (b) is identified as the antibody mimetic that specifically binds to an allosteric site of Aurora A kinase, and

(d) preparing an expression vector comprising a polynucleotide encoding the identified antibody mimetic.