	US 12,253,513 B2
	Methods and compositions for the selective lysis of blood cells and separation of microbial cells
Samad Talebpour, Richmond Hill (CA); Aye Aye Khine, Thornhill (CA); Vilcy Parmar, North York (CA); Sukhdev Manku, Toronto (CA); and Alaleh Samiei, Richmond Hill (CA)
Assigned to QVELLA CORPORATION, Richmond Hill (CA)
Appl. No. 17/057,088
Filed by QVELLA CORPORATION, Richmond Hill (CA)
PCT Filed May 24, 2019, PCT No. PCT/CA2019/050716 § 371(c)(1), (2) Date Nov. 19, 2020, PCT Pub. No. WO2019/222862, PCT Pub. Date Nov. 28, 2019.
Claims priority of provisional application 62/776,126, filed on Dec. 6, 2018.
Claims priority of provisional application 62/676,771, filed on May 25, 2018.
Prior Publication US 2021/0208128 A1, Jul. 8, 2021
Int. Cl. G01N 33/49 (2006.01); G01N 35/00 (2006.01)

CPC G01N 33/491 (2013.01) [G01N 35/00 (2013.01); G01N 2035/00495 (2013.01)]

38 Claims

1. A method of separating microbial cells from a sample, the method comprising:

mixing a blood sample and a blood lysis reagent, the blood lysis reagent comprising saponin, sodium polyanethole sulfonate and an alkaline buffer, to obtain a mixture having a concentration of saponin between 0.75 and 60 mg/ml, a concentration of sodium polyanethole sulfonate between 0.35 and 50 mg/ml and a pH between 7.8 and 10; and

separating microbial cells from the mixture.