US 12,253,482 B2
Method for determining structure of substance in multicomponent sample
Yoshimasa Taniguchi, Tokyo (JP); Mayuka Miwa, Tokyo (JP); Naoya Kitada, Tokyo (JP); Hotaka Sanda, Tokyo (JP); Takayuki Nishimura, Tokyo (JP); and Eriko Takeshi, Tokyo (JP)
Assigned to KIRIN HOLDINGS KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 17/773,645
Filed by KIRIN HOLDINGS KABUSHIKI KAISHA, Tokyo (JP)
PCT Filed Oct. 30, 2020, PCT No. PCT/JP2020/040979
§ 371(c)(1), (2) Date May 2, 2022,
PCT Pub. No. WO2021/085645, PCT Pub. Date May 6, 2021.
Claims priority of application No. 2019-200020 (JP), filed on Nov. 1, 2019; and application No. 2020-014066 (JP), filed on Jan. 30, 2020.
Prior Publication US 2022/0381708 A1, Dec. 1, 2022
Int. Cl. G01N 30/02 (2006.01); G01N 23/20 (2018.01); G01N 23/2005 (2018.01); G01N 30/26 (2006.01); G01N 30/88 (2006.01)
CPC G01N 23/2005 (2013.01) [G01N 30/02 (2013.01); G01N 30/88 (2013.01); G01N 30/26 (2013.01); G01N 2223/0566 (2013.01); G01N 2223/607 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of determining the structure of a target substance contained in a mixture of two or more substances, the method comprising the steps of: (A) isolating the target substance from the mixture by means of supercritical fluid chromatography, (B) soaking the isolated target substance into a crystalline sponge to produce a sample for crystallography, and (C) performing a crystallographic analysis on the sample for crystallography, wherein, in the step (A), a volatile solvent is used as a mobile phase in the supercritical fluid chromatography.