US 12,253,447 B2
Isolation method for isolating target particles from liquid samples
Yuchao Chen, Shenzhen (CN)
Assigned to Shenzhen Huixin Life Technologies Co., Ltd, Shenzhen (CN)
Filed by Shenzhen Huixin Life Technologies Co., Ltd, Shenzhen (CN)
Filed on Dec. 6, 2023, as Appl. No. 18/530,661.
Application 18/530,661 is a division of application No. 17/576,654, filed on Jan. 14, 2022, granted, now 11,874,209.
Application 17/576,654 is a continuation in part of application No. PCT/CN2019/096425, filed on Jul. 17, 2019.
Prior Publication US 2024/0102900 A1, Mar. 28, 2024
Int. Cl. B01L 3/00 (2006.01); B01F 33/302 (2022.01); B01F 33/3033 (2022.01); B01L 7/00 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); G01N 1/34 (2006.01); G01N 1/40 (2006.01); G01N 21/29 (2006.01)
CPC G01N 1/34 (2013.01) [G01N 1/4005 (2013.01); G01N 2001/4016 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method for isolation of target particles from a plurality of liquid samples, the method comprising:
providing a plurality of isolation chips, each of the plurality of isolation chips comprising a sample reservoir, and a first outlet and a second outlet disposed at opposite sides of the sample reservoir;
placing the plurality of isolation chips on a first support platform, and adding the plurality of liquid samples on a second support platform;
rotating the second support platform to enable the plurality of liquid samples to successively move to a second preset position;
rotating the first support platform to enable the plurality of isolation chips to successively move to a first preset position;
rotating at least one sampling member to form a sampling trajectory, the first preset position and the second preset position being on the sampling trajectory; and
controlling each of the at least one sampling member to collect one of the plurality of liquid samples at the second preset position, and to add the collected liquid sample to one of the plurality of isolation chips at the first preset position;
evacuating through the first outlet to generate a negative pressure in each of the plurality of isolation chips;
stopping evacuating through the first outlet;
evacuating through the second outlet to generate a negative pressure in each of the plurality of isolation chips;
stopping evacuating through the second outlet.