| CPC C12Q 1/6813 (2013.01) [C12Q 1/6844 (2013.01)] | 18 Claims |
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1. A complex comprising multiple nucleic acid molecules that are hybridized together, each comprising, from 5′ to 3′:
i. a first complementary sequence;
ii. a spacer sequence; and
iii. a second complementary sequence;
wherein the molecules further comprise:
iv. a 5′ end sequence that is 5′ of the first complementary sequence and terminates in
a 5′ phosphate; and/or
a 3′ end sequence that is 3′ of the second complementary sequence and terminates
in a 3′ hydroxyl;
wherein, in the complex: i. the first complementary sequence of one molecule is directly or indirectly hybridized with the second complementary sequence of another molecule in the complex and ii. the spacer sequence and the 3′ and/or 5′ end sequences are single-stranded, and
wherein the complex is tethered to a cell.
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