US 12,252,728 B2
Systems and methods for measuring liver enzyme levels in blood
Jianhong Pei, Somerville, MA (US); Joseph Bedard, Waltham, MA (US); Yiliyasi Wusimanjiang, West Newton, MA (US); Anthony Florindi, Norfolk, MA (US); and Chung Chang Young, Salem, NH (US)
Assigned to NOVA BIOMEDICAL CORPORATION, Waltham, MA (US)
Appl. No. 17/256,210
Filed by Nova Biomedical Corporation, Waltham, MA (US)
PCT Filed Sep. 17, 2019, PCT No. PCT/US2019/051383
§ 371(c)(1), (2) Date Dec. 27, 2020,
PCT Pub. No. WO2021/054937, PCT Pub. Date Mar. 25, 2021.
Prior Publication US 2021/0363560 A1, Nov. 25, 2021
Int. Cl. C12Q 1/00 (2006.01); C12Q 1/42 (2006.01); C12Q 1/48 (2006.01); G01N 27/327 (2006.01); G01N 27/48 (2006.01)
CPC C12Q 1/005 (2013.01) [C12Q 1/42 (2013.01); C12Q 1/48 (2013.01); G01N 27/3272 (2013.01); G01N 27/48 (2013.01)] 31 Claims
OG exemplary drawing
 
1. A disposable test cartridge for use in a point-of-care analyzer to determine the concentration of one or more liver enzymes in a blood sample, the disposable test cartridge comprising:
a cartridge body having a plurality of chambers wherein each of the plurality of chambers has an opening at a top of the cartridge body and at least one of the plurality of chambers contains a reactant mixture capable of undergoing a chemical reaction when combined with a blood sample forming a reaction solution containing at least one reaction product, the reactant mixture containing chemicals specific for being catalyzed by one of the liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), or gamma-glutamyl transferase (GGT) in the blood sample;
a removable cartridge cover connected to the cartridge body and covering the plurality of chambers, the removable cartridge cover having a capillary-receiving aperture and a capillary element removably insertable into the cartridge cover, the capillary element configured for obtaining a blood sample; and
a test strip module directly connected to the cartridge body and containing at least one analyte test strip configured for receiving a portion of the reaction solution and measuring at least one analyte, the at least one analyte being the at least one reaction product.