US 12,252,727 B2
Method for producing drimanyl acetate compounds
Daniel Solis Escalante, Satigny (CH); Christian Görner, Satigny (CH); Laurent Daviet, Satigny (CH); and Qi Wang, Shanghai (CN)
Assigned to FIRMENICH SA, Satigny (CH)
Appl. No. 17/271,474
Filed by FIRMENICH SA, Satigny (CH)
PCT Filed Oct. 14, 2019, PCT No. PCT/EP2019/077716
§ 371(c)(1), (2) Date Feb. 25, 2021,
PCT Pub. No. WO2020/078871, PCT Pub. Date Apr. 23, 2020.
Claims priority of application No. 18200539 (EP), filed on Oct. 15, 2018.
Prior Publication US 2021/0310031 A1, Oct. 7, 2021
Int. Cl. C12P 7/62 (2022.01); C12P 7/02 (2006.01)
CPC C12P 7/62 (2013.01) [C12P 7/02 (2013.01)] 11 Claims
 
1. A biocatalytic method of producing a drimanyl acetate compound comprising the steps of
(1) contacting in the presence of an acetyl group donor a drimanyl alcohol with a polypeptide having acetyl transferase activity of the enzyme class EC 2.3.1 capable of transferring an acetyl group from said acetyl group donor to said drimanyl alcohol to obtain a drimanyl acetate; and
(2) optionally isolating a drimanyl acetate compound from the reaction product of step (1);
wherein said acetyl group donor is acetyl-Coenzyme A (acetyl-CoA); and
wherein said acetyl transferase is selected from the group consisting of
a) polypeptides comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 9, 17, 118, 124, 144, 23, 21, 11, 19, 13, 15, 25, 121, 143, 127, 130, 133, and 136; and
b) polypeptides having acetyl transferase activity and comprising an amino acid sequence showing a degree of sequence identity of at least 80% to at least one of said amino acid sequences selected from the group consisting of SEQ ID NO: 9, 17, 118, 124, 144, 23, 21, 11, 19, 13, 15, 25, 121, 143, 127, 130, 133, and 136;
wherein the biocatalytic method is performed in an aqueous environment.