US 12,252,724 B2
Biosynthesis of retinoids
Nathalie Balch, Kaiseraugst (CH); Paul Blomquist, Kaiseraugst (CH); Reed Doten, Kaiseraugst (CH); Peter Houston, Kaiseraugst (CH); Ethan Lam, Kaiseraugst (CH); Jenna McMahon, Kaiseraugst (CH); Joshua Trueheart, Kaiseraugst (CH); and Celine Viarouge, Kaiseraugst (CH)
Assigned to DSM IP ASSETS B.V., Heerlen (NL)
Filed by DSM IP ASSETS B.V., Heerlen (NL)
Filed on Jan. 30, 2023, as Appl. No. 18/161,236.
Application 18/161,236 is a continuation of application No. 16/647,730, granted, now 11,578,344, previously published as PCT/EP2018/076033, filed on Sep. 25, 2018.
Claims priority of provisional application 62/562,712, filed on Sep. 25, 2017.
Claims priority of provisional application 62/562,699, filed on Sep. 25, 2017.
Claims priority of provisional application 62/562,672, filed on Sep. 25, 2017.
Claims priority of provisional application 62/562,602, filed on Sep. 25, 2017.
Claims priority of application No. 18168564 (EP), filed on Apr. 20, 2018; and application No. 00715/18 (CH), filed on Jun. 5, 2018.
Prior Publication US 2023/0227862 A1, Jul. 20, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/22 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12P 7/62 (2022.01)
CPC C12P 7/22 (2013.01) [C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12P 7/62 (2013.01); C12Y 101/01105 (2013.01); C12Y 203/0102 (2013.01)] 20 Claims
 
1. A process for production of vitamin A comprising cultivating a retinoid-producing fungal host cell in an aqueous medium under suitable culture conditions, wherein
(a) the host cell expresses
(i) a heterologous beta-carotene oxidizing enzyme (BCO) capable of catalyzing the conversion of beta-carotene into a retinal mix comprising cis- and trans-retinal,
(ii) a heterologous retinol dehydrogenase (RDH) enzyme [EC 1.1.1.105] capable of converting retinal into retinol, and
(iii) a heterologous acetyl transferase 1 enzyme (ATF1) [EC 2.3.1.84] capable of catalysing the conversion of trans-retinol to a retinyl acetate, and
(b) the host cell is capable of
(i) producing a retinal mix comprising cis- and trans-retinal,
(ii) converting the retinal mix into retinol with a total conversion of at least 90% towards generation of retinol, and
(iii) converting the retinol into retinyl acetate, wherein at least 40% of the total amount of retinoids produced by said host cell is retinyl acetate.