US 12,252,711 B2
Osteoporosis model comprising calcium phosphate hydrogel composition and use thereof
Mi Sun Cha, Seoul (KR); and Jung Ju Kim, Ansan-si (KR)
Assigned to MEDIFAB CO., LTD., Seoul (KR)
Appl. No. 17/311,814
Filed by MEDIFAB CO., LTD., Seoul (KR)
PCT Filed Jul. 23, 2020, PCT No. PCT/KR2020/009694
§ 371(c)(1), (2) Date Jun. 8, 2021,
PCT Pub. No. WO2021/015562, PCT Pub. Date Jan. 28, 2021.
Claims priority of application No. 10-2019-0090487 (KR), filed on Jul. 25, 2019; and application No. 10-2019-0090492 (KR), filed on Jul. 25, 2019.
Prior Publication US 2022/0145254 A1, May 12, 2022
Int. Cl. G01N 33/84 (2006.01); A61L 27/52 (2006.01); C12N 5/077 (2010.01); C12N 5/0775 (2010.01); G01N 33/50 (2006.01)
CPC C12N 5/0654 (2013.01) [C12N 5/0662 (2013.01); G01N 33/5038 (2013.01); G01N 33/5088 (2013.01); G01N 33/84 (2013.01); C12N 2501/25 (2013.01); C12N 2502/1142 (2013.01); C12N 2502/1311 (2013.01); C12N 2506/1346 (2013.01); C12N 2513/00 (2013.01); C12N 2533/18 (2013.01); C12N 2533/54 (2013.01); C12N 2533/74 (2013.01); C12N 2537/10 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method of preparing a hydrogel composition comprising:
preparing a first composition by dissolving chitosan in an acidic solution containing gelatin;
preparing a second composition by dispersing calcium phosphate in an acidic solution containing calcium chloride and then conducting sonication;
preparing a mixed solution by mixing the first composition with the second composition; and
preparing a hydrogel composition by adding dropwise the mixed solution into an alkaline solution.