	US 12,252,700 B2
	Paramyxovirus vector
Koichi Saeki, Tokyo (JP); Kohji Kusano, Tokyo (JP); Hiroto Hara, Tokyo (JP); Makoto Inoue, Tokyo (JP); and Jitsutaro Kawaguchi, Tokyo (JP)
Assigned to ID Pharma Co., Ltd., Tokyo (JP)
Appl. No. 15/774,491
Filed by ID Pharma Co., Ltd., Tokyo (JP)
PCT Filed Nov. 4, 2016, PCT No. PCT/JP2016/082821 § 371(c)(1), (2) Date Jan. 8, 2019, PCT Pub. No. WO2017/082174, PCT Pub. Date May 18, 2017.
Claims priority of application No. 2015-223133 (JP), filed on Nov. 13, 2015.
Prior Publication US 2020/0283797 A1, Sep. 10, 2020
Int. Cl. C12N 15/86 (2006.01); C12N 7/00 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/86 (2013.01) [C12N 7/00 (2013.01); C12N 15/1131 (2013.01); C12N 2760/18043 (2013.01)]

2 Claims

1. A modified Sendai virus vector, which comprises two P genes, wherein

a first P gene encodes a first P protein which comprises D433A, R434A, K437A, and L511F substitutions and is temperature sensitive, and further has a degron, and

a second P gene has a target sequence of a microRNA in its coding region or 5′- or 3′-noncoding region, and a P protein encoded by the second P gene does not contain a temperature sensitive mutation and is functionally expressed at 37° C.