	US 12,252,694 B2
	*Genomic editing vector for Eubacterium callanderi, method for editing genome of Eubacterium callanderi* using the same, and transgenic Eubacterium callanderi strains using the same**
In Seop Chang, Gwangju (KR); Ji Yeon Kim, Gwangju (KR); Ji Yeong Jeong, Gwangju (KR); So Young Oh, Gwangju (KR); and Jin Sung Jeon, Gwangju (KR)
Assigned to GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY, Gwangju (KR)
Filed by GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY, Gwangju (KR)
Filed on Jul. 16, 2021, as Appl. No. 17/377,590.
Claims priority of application No. 10-2020-0089015 (KR), filed on Jul. 17, 2020.
Prior Publication US 2022/0017910 A1, Jan. 20, 2022
Int. Cl. C12N 15/74 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/74 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)]

12 Claims

1. A genomic editing vector for Eubacterium callanderi, comprising:

a DNA sequence encoding a guide RNA (gRNA) of a cleavage target gene;

a DNA sequence encoding a Cas9 protein;

a P_rbopromoter that is operably linked to the DNA sequence encoding a Cas9 protein;

a replication starting point derived from E. coli;

a replication starting point for Eubacterium callanderi; and

a marker for selecting transgenic strains.